Direct DNA extraction method of an obligate parasitic fungus from infected plant tissue
, “Direct DNA extraction method of an obligate parasitic fungus from infected plant tissue”, vol. 14, pp. 18546-18551, 2015.
Powdery mildew and rust fungi are obligate parasites that cannot live without host organisms. They are difficult to culture in synthetic medium in the laboratory. Genomic DNA extraction is one of the basic molecular techniques used to study the genetic structure of populations. In this study, 2 different DNA extraction methods, Chelex-100 and cetyltrimethylammonium bromide (CTAB), were used to extract DNA from euonymus powdery mildew and Puccinia striiformis f. sp Tritici.
