Cerrado

Development of microsatellite markers for Hoplias malabaricus (Erythrinidae)

S. G. C. A. Gondim, Resende, L. V., Brondani, R. P. V., Collevatti, R. G., Silva-Júnior, N. J., Pereira, R. R., and Telles, M. P. C., Development of microsatellite markers for Hoplias malabaricus (Erythrinidae), vol. 9. pp. 1513-1517, 2010.

We identified 14 microsatellite loci for the wolf fish, Hoplias malabaricus (Erythrinidae), from a genomic shotgun library. Twenty-five primers were designed, and 48 individuals of H. malabaricus from four localities of northwest Goiás, in central Brazil, were genotyped to characterize the polymorphism at each locus. Fourteen primers amplified clearly interpretable products using a single PCR protocol; six loci were polymorphic, but with a low number of alleles per locus (2 or 3). Expected heterozygosities for polymorphic loci ranged from 0.136 to 0.505.

Isolation and characterization of microsatellite markers in the armored catfish Hypostomus gymnorhynchus (Loricariidae)

M. P. C. Telles, Resende, L. V., Brondani, R. P. V., Collevatti, R. G., Costa, M. C., and Júnior, N. J. Silva, Isolation and characterization of microsatellite markers in the armored catfish Hypostomus gymnorhynchus (Loricariidae), vol. 9. pp. 1770-1774, 2010.

We isolated and characterized 10 microsatellite loci in the armored catfish (Hypostomus gymnorhynchus, Loricariidae), using a genomic shotgun library to obtain the repetitive sequences. Twenty-four primers were designed and 14 individuals of H. gymnorhynchus from the Caiapó River, in central Brazil, were genotyped using these primers to analyze the polymorphism at each locus. All loci showed low polymorphism, with a low number of alleles per locus (1 or 2), except locus Hg_E19, which had 11 alleles.

Leaf age affects the quality of DNA extracted from Dimorphandra mollis (Fabaceae), a tropical tree species from the Cerrado region of Brazil

P. A. Moreira and Oliveira, D. A., Leaf age affects the quality of DNA extracted from Dimorphandra mollis (Fabaceae), a tropical tree species from the Cerrado region of Brazil, vol. 10, pp. 353-358, 2011.

Isolation of high-quality DNA from plants, especially plants from the Cerrado, is notoriously difficult because of polysaccharides and secondary compounds produced by plants from this biome. DNA isolation and its quality may be compromised by chemical defenses such as tannins and phenols. Quantitative plant defenses tend to have a cumulative effect, increasing in concentration during leaf development, reducing DNA quality extracted in mature compared to young leaves. We report the effect of leaf age on DNA extraction of Dimorphandra mollis.

Development of microsatellite markers for the endangered Neotropical tree species Tibouchina papyrus (Melastomataceae)

M. P. C. Telles, Peixoto, F. P., Lima, J. S., Resende, L. V., Vianello, R. P., Walter, M. E. M. T., and Collevatti, R. G., Development of microsatellite markers for the endangered Neotropical tree species Tibouchina papyrus (Melastomataceae), vol. 10, pp. 321-325, 2011.

We isolated and characterized 12 microsatellite loci for Tibouchina papyrus (Melastomataceae), an endangered species with narrow and disjunct range, endemics to a few localities in “cerrado rupestre” from Central Brazil. These microsatellites were obtained by sequencing of a genomic shotgun library for primer design. Leaves from 96 individuals collected in the three known local populations were genotyped using the 12 primers designed to analyze the polymorphisms at each locus. The number of alleles per locus ranged from one to six; two loci were monomorphic.

Genetic population structure and hybridization in two sibling species, Tomoplagia reticulata and Tomoplagia pallens (Diptera: Tephritidae)

A. G. Abreu and Solferini, V. N., Genetic population structure and hybridization in two sibling species, Tomoplagia reticulata and Tomoplagia pallens (Diptera: Tephritidae), vol. 7, pp. 1298-1311, 2008.

Tomoplagia reticulata and T. pallens are sibling species that are specialists on Eremanthus glomerulatus. Besides adult terminalia, they show slight morphological differences and distinct geographic distributions. Once, however, they were found sympatrically. Using data from allozyme and mtDNA, we examined patterns of intra- and interspecific genetic structure, and investigated the possible occurrence of gene flow between them. Both species showed low diversity and high genetic structure, which can be linked to their high degree of specialization.

Transferability of short tandem repeat markers for two wild Canid species inhabiting the Brazilian Cerrado

F. M. Rodrigues, Telles, M. P. C., Resende, L. V., Soares, T. N., Diniz-Filho, J. A. F., Jácomo, A. T. A., and Silveira, L., Transferability of short tandem repeat markers for two wild Canid species inhabiting the Brazilian Cerrado, vol. 5. pp. 846-850, 2006.

The maned wolf (Chrysocyon brachyurus) and the crab-eating fox (Cerdocyon thous) are two wild-canid species found in the Brazilian Cerrado. We tested cross-amplification and transferability of 29 short tandem repeat primers originally developed for cattle and domestic dogs and cats on 38 individuals of each of these two species, collected in the Emas National Park, which is the largest national park in the Cerrado region.

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