Cellulolytic activity

Evaluation of cellulolytic activity in insect digestive fluids

L. - J. Su, Zhang, H. - F., Yin, X. - M., Chen, M., Wang, F. - Q., Xie, H., Zhang, G. - Z., and Song, A. - D., Evaluation of cellulolytic activity in insect digestive fluids, vol. 12, pp. 2432-2441, 2013.

Efficient and low-cost cellulolytic enzymes are urgently needed to degrade recalcitrant plant biomass during the industrial production of lignocellulosic biofuels. Here, the cellulolytic activities in the gut fluids of 54 insect species that belong to 7 different taxonomic orders were determined using 2 different substrates, carboxymethyl cellulose (CMC) (approximating endo-β-1,4-glucanase) and filter paper (FP) (total cellulolytic activities). The use of CMC as the substrate in the zymogram analysis resulted in the detection of distinct cellulolytic protein bands.

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