Cell therapy

Generation of bovine (Bos indicus) and buffalo (Bubalus bubalis) adipose tissue derived stem cells: isolation, characterization, and multipotentiality

R. V. Sampaio, Chiaratti, M. R., Santos, D. C. N., Bressan, F. F., Sangalli, J. R., Sá, A. L. A., Silva, T. V. G., Costa, N. N., Cordeiro, M. S., Santos, S. S. D., Ambrósio, C. E., Adona, P. R., Meirelles, F. V., Miranda, M. S., and Ohashi, O. M., “Generation of bovine (Bos indicus) and buffalo (Bubalus bubalis) adipose tissue derived stem cells: isolation, characterization, and multipotentiality”, vol. 14, pp. 53-62, 2015.

Adult stem cells are known for their plasticity and their potential to differentiate into several different cell types; these characteristics have implications for cell therapy and reproductive biotechnologies. In this study, we report on the isolation and characterization of mesenchymal stem cells (MSC) derived from bovine and buffalo adipose tissue. Cells isolated using enzymatic digestion of bovine and buffalo adipose-tissue biopsy samples were grown in vitro for at least 15 passages, verifying their capacity to proliferate.

Therapeutic potential of mesenchymal stem cells to treat Achilles tendon injuries

M. H. C. Vieira, Oliveira, R. J., Eça, L. P. M., Pereira, I. S. O., Hermeto, L. C., Matuo, R., Fernandes, W. S., Silva, R. A., and Antoniolli, A. C. M. B., “Therapeutic potential of mesenchymal stem cells to treat Achilles tendon injuries”, vol. 13, pp. 10434-10449, 2014.

Rupture of the Achilles tendon diminishes quality of life. The gold-standard therapy is a surgical suture, but this presents complications, including wound formation and inflammation. These complications spurred evaluation of the therapeutic potential of mesenchymal stem cells (MSCs) from adipose tissue. New Zealand rabbits were divided into 6 groups (three treatments with two time points each) evaluated at either 14 or 28 days after surgery: cross section of the Achilles tendon (CSAT); CSAT + Suture; and CSAT + MSC.

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