Cell proliferation

MicroRNA-21 promotes proliferation of rat hepatocyte BRL-3A by targeting FASLG

J. J. Li, Chan, W. H., Leung, W. Y., Wang, Y., and Xu, C. S., “MicroRNA-21 promotes proliferation of rat hepatocyte BRL-3A by targeting FASLG”, vol. 14, pp. 4150-4160, 2015.

Rat liver regeneration (RLR) induced by partial hepatectomy involves cell proliferation regulated by numerous factors, including microRNAs (miRNAs). miRNA high-throughput sequencing has been established and used to analyze miRNA expression profiles. This study showed that 39 miRNAs were related to RLR through the analysis of miRNA high-throughput sequencing. Their role toward rat normal hepatocyte line BRL-3A was studied by gain- and loss-of-function analyses, and one of them, microRNA-21 (miR-21), obviously upregulated and promoted BRL-3A cell proliferation.

Expression and role of cyclophilin B in stomach cancer

D. Q. Meng, Li, P. L., and Xie, M., “Expression and role of cyclophilin B in stomach cancer”, vol. 14, pp. 5346-5354, 2015.

We elucidated the expression of ciclosporin protein B (cyclophilin B) in stomach cancer tissue and the correlation between cyclophilin B and clinicopathological parameters, and determined the effect of cyclophilin B on growth and proliferation of stomach cancer cells.

FOXO1 is a tumor suppressor in cervical cancer

B. Zhang, Gui, L. S., Zhao, X. L., Zhu, L. L., and Li, Q. W., “FOXO1 is a tumor suppressor in cervical cancer”, vol. 14, pp. 6605-6616, 2015.

Forkhead box protein O1 (FOXO1) is an important transcriptional regulator of cell proliferation, and is considered essential for tumor growth and progression. However, the function of FOXO1 in human cervical cancer remains unclear. In this study, we investigated the role of FOXO1 in cervical cancer. Our results showed that FOXO1 expression was lower in cervical cancer than in cervical intraepithelial neoplasia and normal cervix by immunohistochemical analysis (P 2/M phase and upregulation of caspases-3 and -9 gene expression.

Identification and characterization of microRNAs expressed in chicken skeletal muscle

A. P. D. Andreote, Rosário, M. F., Ledur, M. C., Jorge, E. C., Sonstegard, T. S., Matukumalli, L., and Coutinho, L. L., “Identification and characterization of microRNAs expressed in chicken skeletal muscle”, vol. 13, pp. 1465-1479, 2014.

MicroRNAs (miRNAs, miRs) encompass a class of small non-coding RNAs that often negatively regulate gene expression. miRNAs play an essential role in skeletal muscle, determining the proper development and maintenance of this tissue. In comparison to other organs and tissues, the full set of muscle miRNAs and its expression patterns are still poorly understood. In this report, a chicken skeletal muscle miRNA library was constructed, and the expression of selected miRNAs was further characterized during muscle development in chicken lines with distinct muscling phenotypes.

Cell proliferation and apoptosis in the fetal and neonatal ovary of guinea pigs

S. Y. Sun, Zhang, W., Han, X., Huang, R. H., and Shi, F. X., “Cell proliferation and apoptosis in the fetal and neonatal ovary of guinea pigs”, vol. 13, pp. 1570-1578, 2014.

The guinea pig is an excellent animal model for studying reproductive biology of adult humans and most domestic animals. Yet, whether this animal might serve as a good model for embryonic stage investigations and determinations of signals affecting or directing ovary development remains unknown. These questions were addressed by examining morphological evolution and the expression of biomarkers of cell proliferation and apoptosis in the ovaries of fetal and neonatal guinea pigs in the present study.

MTA1 promotes cell proliferation via DNA damage repair in epithelial ovarian cancer

Q. Y. Yang, Li, J. H., Wang, Q. Y., Wu, Y., Qin, J. L., Cheng, J. J., and Qiu, J., “MTA1 promotes cell proliferation via DNA damage repair in epithelial ovarian cancer”, vol. 13, pp. 10269-10278, 2014.

We examined whether metastasis-associated gene 1 (MTA1) promotes cell proliferation via DNA damage repair in ovarian cancer. MTA1 was successfully down-regulated using small interfering RNA in the epithelial ovarian cancer cell lines SKOV-3 and OVCAR-3. Cell growth was evaluated through MTT and colony formation assays. Fluorescence-activated cell sorting analysis was used to evaluate the distribution of cells in the cell cycle, and cytotoxicity assays were performed to study cell sensitivity to cisplatin.

Knockdown expression of Apc11 leads to cell-cycle distribution reduction in G2/M phase

Y. - J. Shi and Huo, K. - K., “Knockdown expression of Apc11 leads to cell-cycle distribution reduction in G2/M phase”, vol. 11, pp. 2814-2822, 2012.

Anaphase-promoting complex/cyclosome (APC/C) is a key E3 ubiquitin ligase in cell division, which catalyses ubiquitination of cell-cycle regulators. Studying this complex could reveal important information regarding its application in cancer research and therapy. In this study, 4 synthesized small interfering RNAs (siRNAs) were transfected into HEK293T cells to suppress messenger RNA (mRNA) of Apc11; 2 of these reduced the amount of Apc11 mRNA by over 50%.

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