Cell isolation

Isolation of transfected fibroblast clones for use in nuclear transfer and transgene detection in cattle embryos

E. O. Melo, Sousa, R. V., Iguma, L. T., Iguma, L. T., Franco, M. M., Rech, E. L., Rech, E. L., and Rumpf, R., Isolation of transfected fibroblast clones for use in nuclear transfer and transgene detection in cattle embryos, vol. 4, pp. 812-821, 2005.

Transgenesis in cattle has provided numerous opportunities for livestock production. The development of nuclear transfer (NT) technology has improved the production of transgenic livestock. However, the isolation of pure colonies from a single transfection event remains laborious and can be a constraint in the production of transgenic livestock. We used 96-well cell culture plates to isolate cell lineages obtained from a single fibroblast transfected with the pCi-Neo plasmid.

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