cDNA library

Expressed sequence tag analysis of functional genes associated with adventitious rooting in Liriodendron hybrids

Y. D. Zhong, Sun, X. Y., Liu, E. Y., Li, Y. Q., Gao, Z., Yu, F. X., Zhong, Y. D., Sun, X. Y., Liu, E. Y., Li, Y. Q., Gao, Z., and Yu, F. X., “Expressed sequence tag analysis of functional genes associated with adventitious rooting in Liriodendron hybrids”, vol. 15, p. -, 2016.

Liriodendron hybrids (Liriodendron chinense x L. tulipifera) are important landscaping and afforestation hardwood trees. To date, little genomic research on adventitious rooting has been reported in these hybrids, as well as in the genus Liriodendron. In the present study, we used adventitious roots to construct the first cDNA library for Liriodendron hybrids. A total of 5176 expressed sequence tags (ESTs) were generated and clustered into 2921 unigenes.

Biological characterization of liver fatty acid binding gene from miniature pig liver cDNA library

Y. H. Gao, Wang, K. F., Zhang, S., Fan, Y. N., Guan, W. J., and Ma, Y. H., “Biological characterization of liver fatty acid binding gene from miniature pig liver cDNA library”, vol. 14, pp. 9764-9771, 2015.

Liver fatty acid binding proteins (L-FABP) are a family of small, highly conserved, cytoplasmic proteins that bind to long-chain fatty acids and other hydrophobic ligands. In this study, a full-length enriched cDNA library was successfully constructed from Wuzhishan miniature pig, and then the L-FABP gene was cloned from this cDNA library and an expression vector (pEGFP-N3-L-FABP) was constructed in vitro. This vector was transfected into hepatocytes to test its function.

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