cDNA library

Biological characterization of liver fatty acid binding gene from miniature pig liver cDNA library

Y. H. Gao, Wang, K. F., Zhang, S., Fan, Y. N., Guan, W. J., and Ma, Y. H., “Biological characterization of liver fatty acid binding gene from miniature pig liver cDNA library”, vol. 14, pp. 9764-9771, 2015.

Liver fatty acid binding proteins (L-FABP) are a family of small, highly conserved, cytoplasmic proteins that bind to long-chain fatty acids and other hydrophobic ligands. In this study, a full-length enriched cDNA library was successfully constructed from Wuzhishan miniature pig, and then the L-FABP gene was cloned from this cDNA library and an expression vector (pEGFP-N3-L-FABP) was constructed in vitro. This vector was transfected into hepatocytes to test its function.

Construction and analysis of a suppression subtractive hybridization library of regeneration-related genes in soybean

J. Sun, Li, J., Liu, M., Zhang, B. B., Li, D. M., Wang, M., Zhang, C., Li, W. B., Su, A. Y., and Wu, X. X., “Construction and analysis of a suppression subtractive hybridization library of regeneration-related genes in soybean”, vol. 14, pp. 763-773, 2015.

The development of a genetic transformation system is needed to address the problem of the low efficiency associated with soybean regeneration. To contribute to the enhancement of the soybean regenerative capacity, we explored the developmental mechanisms of soybean regeneration at the molecular level using a suppression subtractive hybridization cDNA library constructed from cotyledonary nodes of soybean cultivar DN50. A total of 918 positive clones were identified and screened, with most inserted fragments ranging from 100 to 750 bp.

Transcript profiling of expressed sequence tags from semimembranosus muscle of commercial and naturalized pig breeds

C. S. Nascimento, Peixoto, J. O., Verardo, L. L., Campos, C. F., Weller, M. M. C., Faria, V. R., Botelho, M. E., Martins, M. F., Machado, M. A., Silva, F. F., Lopes, P. S., and Guimarães, S. E. F., “Transcript profiling of expressed sequence tags from semimembranosus muscle of commercial and naturalized pig breeds”, vol. 11, pp. 3315-3328, 2012.

In general, genetic differences across different breeds of pig lead to variation in mature body size and slaughter age. The Commercial breeds Duroc and Large White and the local Brazilian breed Piau are ostensibly distinct in terms of growth and muscularity, commercial breeds are much leaner while local breeds grow much slower and are fat type pigs. However, the genetic factors that underlie such distinctions remain unclear. We used expressed sequence tags (ESTs) to characterize and compare transcript profiles in the semimembranosus muscle of these pig breeds.

Construction of a cDNA library of the Chinese wild Vitis amurensis under cold stress and analysis of potential hardiness-related expressed sequence tags

J. Zhang, Liu, N., Niu, R., Liu, Y., Zhai, H., Xu, W., and Wang, Y., “Construction of a cDNA library of the Chinese wild Vitis amurensis under cold stress and analysis of potential hardiness-related expressed sequence tags”, vol. 12, pp. 1182-1193, 2013.

A cDNA library of Chinese wild Vitis amurensis, which is the most cold-resistant species in the genus Vitis, was constructed using young leaves of seedlings of the clone Heilongjiang potted and subjected to cold stress. The leaves were harvested at various times after cold stress for total RNA extraction, which was used to generate expressed sequence tags (ESTs). The titer of the original library was 2.67 x 106 pfu/mL and the corresponding combination frequency was 98.5%.

Expressed sequenced tags profiling of resistant and susceptible Gyr x Holstein cattle infested with the tick Rhipicephalus (Boophilus) microplus

C. S. Nascimento, Machado, M. A., Guimarães, S. E. F., Martins, M. F., Peixoto, J. O., Furlong, J., Prata, M. C. A., Verneque, R. S., Teodoro, R. L., and Lopes, P. S., “Expressed sequenced tags profiling of resistant and susceptible Gyr x Holstein cattle infested with the tick Rhipicephalus (Boophilus) microplus”, vol. 10, pp. 3803-3816, 2011.

Tick resistance in cattle is mainly found in zebu (Bos indicus) animals, although it is also present in some taurine (B. taurus) breeds. In order to characterize functional genes involved in tick resistance/susceptibility in cattle, two cDNA libraries were generated using skin tissues of selected Holstein x Gyr animals. A total of 2700 high-quality reads from both resistant and susceptible cDNA were assembled into 458 sequences (contigs) and 834 singletons, with a mean size of 447.7 nucleotides.

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