cDNA-AFLP

cDNA-AFLP transcriptional profiling reveals genes expressed during flower development in Oncidium Milliongolds

X. Qian, Gong, M. J., Wang, C. X., and Tian, M., cDNA-AFLP transcriptional profiling reveals genes expressed during flower development in Oncidium Milliongolds, vol. 13, pp. 6303-6315, 2014.

The flower developmental process, which is crucial to the whole lifecycle of higher plants, is influenced by both environmental and endogenous factors. The genus Oncidium is commercially important for cut flower and houseplant industry and is ideal for flower development studies. Using cDNA-amplified restriction fragment length polymorphism analysis, we profiled transcripts that are differentially expressed during flower development of Oncidium Milliongolds. A total of 15,960 transcript-derived fragments were generated, with 114 primer sets.

Identification of differentially expressed genes involved in early bolting of Angelica sinensis (Apiaceae)

G. Yu, Ma, Y. X., Duan, J. - A., Song, B. S., and He, Z. Q., Identification of differentially expressed genes involved in early bolting of Angelica sinensis (Apiaceae), vol. 11, pp. 494-502, 2012.

Angelica sinensis is a highly valued medicinal herb, known as female ginseng that is widely cultivated in China. Although A. sinensis is in great demand due to its multiple medicinal and food applications, its early bolting rate (almost 40%) seriously affects crop quality. To better understand its flowering mechanism, cDNA-amplified RFLP analysis was employed to look for gene expression differences between flower bud and shoot apical meristem tissues. Sixty-four primer sets were used, with each primer set amplified to 60 transcript-derived fragments.

Analysis of differential gene expression during floral bud abortion in radish (Raphanus sativus L.)

J. Zhang, Sun, X. L., Zhang, L. G., Hui, M. X., and Zhang, M. K., Analysis of differential gene expression during floral bud abortion in radish (Raphanus sativus L.), vol. 12, pp. 2507-2516, 2013.

Radish floral bud abortion (FBA) is an adverse biological phenomenon that occurs during reproduction. Although FBA occurs frequently, its mechanism remains unknown. To elucidate the molecular mechanism underlying FBA, we detected gene expression differences between aborted and normal buds of radish using cDNA-amplified fragment length polymorphism (AFLP) and real-time polymerase chain reaction (real-time PCR).

Isolation of fertility-related genes of multiple-allele-inherited male sterility in Brassica rapa ssp pekinensis by cDNA-AFLP

R. Q. Ji, Song, Q., Xin, X. F., Zhou, X., and Feng, H., Isolation of fertility-related genes of multiple-allele-inherited male sterility in Brassica rapa ssp pekinensis by cDNA-AFLP, vol. 10, pp. 4073-4083, 2011.

To better understand the molecular mechanisms of multiple-allele-inherited male sterility in Chinese cabbage (Brassica rapa ssp pekinensis), differentially expressed genes in fertile and sterile plants must be isolated. We used cDNA-AFLP analysis to isolate differentially expressed genes in fertile and sterile buds of the two-type line, AB01. Sixteen high-quality sequences were generated, 11 of which were up-regulated in fertile buds, and five of which were up-regulated in sterile buds.

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