Cattle

Generation of bovine (Bos indicus) and buffalo (Bubalus bubalis) adipose tissue derived stem cells: isolation, characterization, and multipotentiality

R. V. Sampaio, Chiaratti, M. R., Santos, D. C. N., Bressan, F. F., Sangalli, J. R., Sá, A. L. A., Silva, T. V. G., Costa, N. N., Cordeiro, M. S., Santos, S. S. D., Ambrósio, C. E., Adona, P. R., Meirelles, F. V., Miranda, M. S., and Ohashi, O. M., Generation of bovine (Bos indicus) and buffalo (Bubalus bubalis) adipose tissue derived stem cells: isolation, characterization, and multipotentiality, vol. 14, pp. 53-62, 2015.

Adult stem cells are known for their plasticity and their potential to differentiate into several different cell types; these characteristics have implications for cell therapy and reproductive biotechnologies. In this study, we report on the isolation and characterization of mesenchymal stem cells (MSC) derived from bovine and buffalo adipose tissue. Cells isolated using enzymatic digestion of bovine and buffalo adipose-tissue biopsy samples were grown in vitro for at least 15 passages, verifying their capacity to proliferate.

Characterization of a novel CAPN3 transcript generated by alternative splicing in cattle

S. Y. Liu, Jiang, H., Yuan, B., Gao, Y., Dai, L. S., and Zhang, J. B., Characterization of a novel CAPN3 transcript generated by alternative splicing in cattle, vol. 14, pp. 457-463, 2015.

Calpain-3 (CAPN3) is a member of the calpain family of Ca2+-regulated cysteine proteases, which play an important role in sarcomere remodeling and mitochondrial protein turnover, and thus, regulating beef tenderness in cattle. Currently, multiple CAPN3 transcripts have been detected in human, monkey, rat, and rabbit. However, whether this transcript is present in cattle remains unknown. In this study, we identified 2 CAPN3 transcripts in the skeletal muscle individuals of local black cattle from Jilin, China.

Cross-reactivity of anti-human cytokine monoclonal antibodies used as a tool to identify novel immunological biomarkers in domestic ruminants

E. M. S. Dorneles, Araújo, M. S. S., Teixeira-Carvalho, A., Martins-Filho, O. A., and Lage, A. P., Cross-reactivity of anti-human cytokine monoclonal antibodies used as a tool to identify novel immunological biomarkers in domestic ruminants, vol. 14, pp. 940-951, 2015.

Eleven commercially available PE-labeled anti-human (IL-1-α, IL-6, IL-8, TNF-α, IL-17A, IL-5, IL-10, IL-12 and IL-13) and anti-mouse (IL-10, TNF-α) cytokine monoclonal antibodies (mAbs) were tested for cross-reactivity with cattle, goat, and sheep cytokines. Cross-reactivity was assessed by comparative analysis with the standard reactivity of the target species. Our data demonstrated that anti-human IL-1-α, IL-6, IL-8, IL-17A and IL-10 mAbs cross-react with all ruminant species tested.

DNA sequence polymorphism within the bovine adenosine monophosphate deaminase 1 (AMPD1) is associated with production traits in Chinese cattle

C. - B. Wei, Wang, J. - Q., Chen, F. - Y., Niu, H., and Li, K., DNA sequence polymorphism within the bovine adenosine monophosphate deaminase 1 (AMPD1) is associated with production traits in Chinese cattle, vol. 14, pp. 1025-1033, 2015.

The objectives of the present study were to detect an 18-bp deletion mutation in the bovine adenosine monophosphate deaminase 1 (AMPD1) gene and analyze its effect on growth traits in 2 Chinese cattle breeds using DNA sequencing and agarose electrophoresis. The five 19-bp polymerase chain reaction products of the AMPD1 gene exhibited 3 genotypes and 2 alleles: WW: homozygote genotype (wild-type); DD: homozygote genotype (mutant-type); WD: heterozygote genotype. Frequencies of the W allele varied from 66.15-70.35%.

Investigation of TG gene variants and their effects on growth, carcass composition, and meat quality traits in Chinese steers

L. P. Zhang, Gan, Q. F., Hou, G. Y., Gao, H. J., Li, J. Y., and Xu, S. Z., Investigation of TG gene variants and their effects on growth, carcass composition, and meat quality traits in Chinese steers, vol. 14, pp. 5320-5326, 2015.

Thyroid hormones play an important role in regulating metabolism and can affect metabolism-related traits such as fat deposition. The thyroglobulin (TG) gene produces the precursor of thyroid hormones and has been proposed as a candidate gene for a quantitative trait locus with an effect on fat deposition. In this study, we identified 4 novel single nucleotide polymorphisms (SNPs) in the 5' flanking region of the TG gene using a DNA sequencing method.

MULTINDELS-BOV: Zebu traceback method based on DNA insertion-deletion polymorphisms

M. Groenner-Penna, Croce, E. F. D., Pimenta, C. G., Bicalho, H. M. S., and Pena, S. D. J., MULTINDELS-BOV: Zebu traceback method based on DNA insertion-deletion polymorphisms, vol. 13, pp. 9171-9186, 2014.

Brazil is a major producer and exporter of beef, with a herd of approximately 210 million animals. For the meat industry, a reliable animal traceback from its origin to the consumer market is paramount. Of all available identification systems, DNA is the only one that survives the slaughterhouse and reaches the dish of the consumer. DNA polymorphisms are already used for cattle traceback, but primarily for the subspecies Bos taurus taurus. However, in Brazil, another subspecies, B. taurus indicus predominates.

Genetic diversity of Y-short tandem repeats in chinese native cattle breeds

Y. P. Xin, Zan, L. S., Liu, Y. F., Tian, W. Q., Wang, H. B., Cheng, G., Li, A. N., and Yang, W. C., Genetic diversity of Y-short tandem repeats in chinese native cattle breeds, vol. 13, pp. 9578-9587, 2014.

The aim of this study is to use Y-chromosome gene polymorphism method to investigate regional differences in genetic variation and population evolution history of the Chinese native cattle breeds. Six Y-chromosome short tandem repeat (Y-STR) loci (UMN0929, UMN0108, UMN0920, INRA124, UMN2404, and UMN0103) were analyzed using 1016 healthy and heterogenetic males and 90 females of 9 native cattle breeds (Qinchuan, Jinnan, Zaosheng, Luxi, Nanyang, Jiaxian, Dabieshan, Yanbian, and Menggu) in China.

Estimation of taurindicine hybridization of American Zebu cattle in Brazil

P. Ripamonte, Baccaglini, M., César, A. S. M., César, A. S. M., Figueiredo, L. G. G., Balieiro, J. C. C., Caetano, A. R., and Meirelles, F. V., Estimation of taurindicine hybridization of American Zebu cattle in Brazil, vol. 11, pp. 393-403, 2012.

Our objective was to estimate Bos primigenius taurus introgression in American Zebu cattle. One hundred and four American Zebu (Nellore) cattle were submitted to mtDNA, microsatellite and satellite analysis. Twenty-three alleles were detected in microsatellite analysis, averaging 4.6 ± 1.82/locus. Variance component comparisons of microsatellite allele sizes allowed the construction of two clusters separating taurus and indicus. No significant variation was observed when indicus and taurus mtDNA were compared.

Sequence variants in the bovine PRDM16 gene associated with body weight in Chinese cattle breeds

J. Wang, Wang, C., Tian, R., Huang, Y. - Z., Lai, X. - S., Lan, X. - Y., Wang, J. - Q., and Chen, H., Sequence variants in the bovine PRDM16 gene associated with body weight in Chinese cattle breeds, vol. 11, pp. 746-755, 2012.

As a zinc-finger protein, PR domain containing 16 (PRDM16) controls brown fat determination by stimulating brown fat cell production while suppressing the expression of genes for production of white fat cells; mutations in this domain are associated with myelodysplastic syndrome and leukemogenesis. In our study, polymorphisms in exons 2, 3, 4, 5, 7, 8, and 9 of the PRDM16 gene were detected by PCR-SSCP, DNA sequencing and CRS-PCR-RFLP methods in 1031 cattle of the Chinese breeds: Jiaxian, Nanyang, Qinchuan, and Chinese Holstein.

Identification of ANAPC13 gene polymorphisms associated with body measurement traits in Bos taurus

B. J. Jiang, Zhan, X. L., Fu, C. Z., Wang, H. B., Cheng, G., and Zan, L. S., Identification of ANAPC13 gene polymorphisms associated with body measurement traits in Bos taurus, vol. 11, pp. 2862-2870, 2012.

Body measurement traits, influenced by genes and environmental factors, play numerous important roles in the value assessment of productivity and economy. There has been some indication that ANAPC13 influences adult height. We used PCR-SSCP and DNA sequencing technology to identify polymorphisms in the ANAPC13 gene. A polymorphism in intron 1 (A > G at base 17) was identified and an additional polymorphic site (C > T at base 42) was also uncovered, which accompanied the previous polymorphism in more than 98% of the subjects.

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