κ-casein

Duplication polymorphisms in exon 4 of κ-casein gene in yak breeds/populations

S. Pingcuo, Gao, J., Jiang, Z. R., Jin, S. Y., Fu, C. Y., Liu, X., Huang, L., and Zheng, Y. C., Duplication polymorphisms in exon 4 of κ-casein gene in yak breeds/populations, vol. 14, pp. 10242-10248, 2015.

The objective of this study was to compare 12 bp-duplication polymorphisms in exon 4 of the κ-casein gene among 3 breeds/populations of yak (Bos grunniens). Genomic DNA was extracted from yak blood or muscle samples (N = 211) and a partial sequence of exon 4 of κ-casein gene was amplified by polymerase chain reaction. A polyacrylamide gel electrophoresis assay of the products (169 bp) revealed 2 variants. These variants differed in a 12-bp duplication of the nucleotide sequence corresponding to amino acids 147-150 (Glu-Ala-Ser-Pro) or 148-151 (Ala-Ser-Pro-Glu).

Subscribe to κ-casein