Capra hircus

Low diversity of the major histocompatibility complex class II DRA gene in domestic goats (Capra hircus) in Southern China

L. P. Chen, E, G. X., Zhao, Y. J., Na, R. S., Zhao, Z. Q., Zhang, J. H., Ma, Y. H., Sun, Y. W., Zhong, T., Zhang, H. P., and Huang, Y. F., Low diversity of the major histocompatibility complex class II DRA gene in domestic goats (Capra hircus) in Southern China, vol. 14. pp. 6925-6928, 2015.

DRA encodes the alpha chain of the DR heterodimer, is closely linked to DRB and is considered almost monomorphic in major histocompatibility complex region. In this study, we identified the exon 2 of DRA to evaluate the immunogenetic diversity of Chinese south indigenous goat. Two single nucleotide polymorphisms in an untranslated region and one synonymous substitution in coding region were identified. These data suggest that high immunodiversity in native Chinese population.

Single nucleotide polymorphisms in candidate genes associated with gastrointestinal nematode infection in goats

F. A. Bressani, Tizioto, P. C., Giglioti, R., Meirelles, S. L. C., Coutinho, R., Benvenuti, C. L., Malagó-Jr., W., Mudadu, M. A., Vieira, L. S., Zaros, L. G., Carrilho, E., and Regitano, L. C. A., Single nucleotide polymorphisms in candidate genes associated with gastrointestinal nematode infection in goats, vol. 13. pp. 8530-8536, 2014.

Cytokines are small cell-signaling proteins that play an important role in the immune system, participating in intracellular communication. Four candidate genes of the cytokine family (IL2, IL4, IL13, and IFNG) were selected to identify Single Nucleotide Polymorphisms (SNPs) that might be associated with resistance to gastrointestinal endoparasites in goats.

Identification of naturalized goat breeds under conservation from northeastern Brazil using chromosomal markers

R. P. Santos, Affonso, P. R. A. M., Diniz, D., Medrado, A. S., Silva, K. M., and Carneiro, P. L. S., Identification of naturalized goat breeds under conservation from northeastern Brazil using chromosomal markers, vol. 12, pp. 3193-3200, 2013.

In order to provide the first cytogenetic data of naturalized and threatened goat breeds from northeastern Brazil, cytogenetic analyses were carried out in individuals of Repartida and Moxotó breeds raised in Bahia and Ceará States. Males and females of both breeds had 2n = 60, with 29 autosomal acrocentric pairs plus the sex chromosome pair. The number of nucleolar organizer region (NOR)-bearing chromosomes ranged from 6 to 8 per metaphase in Moxotó and Repartida goats, respectively.

Analysis of protein expression and a new prokaryotic expression system for goat (Capra hircus) spermadhesin Bdh-2 cDNA

J. B. Cajazeiras, Melo, L. M., Albuquerque, E. S., Rádis-Baptista, G., Cavada, B. S., and Freitas, V. J. F., Analysis of protein expression and a new prokaryotic expression system for goat (Capra hircus) spermadhesin Bdh-2 cDNA, vol. 8, pp. 1147-1157, 2009.

Low purification efficiency and incomplete characteriza­tion of male goat (buck) spermadhesins (Bdhs) prompted us to develop an effective system to produce recombinant Bdhs (rBdhs). Bdh-2 cDNA was inserted into a prokaryotic expression plasmid, pTrcHis TOPO. The pTrcHis-Bdh-2 system was constructed to produce a His6 fusion protein in Escherichia coli Top10 cells. Recombinant clones were se­lected by growth in ampicillin-enriched medium, PCR amplification and nucleotide sequencing.

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