Buffalo

Synonymous single nucleotide polymorphisms in the MC4R gene that are significantly associated with milk production traits in water buffaloes

T. X. Deng, Pang, C. Y., Liu, M. Q., Zhang, C., Liang, X. W., Deng, T. X., Pang, C. Y., Liu, M. Q., Zhang, C., and Liang, X. W., “Synonymous single nucleotide polymorphisms in the MC4R gene that are significantly associated with milk production traits in water buffaloes”, vol. 15, p. -, 2016.

Melanocortin-4 receptor (MC4R) is associated with feed intake, growth, fatness, and carcass composition in many domestic animals. The aim of this study was to evaluate the association of single nucleotide polymorphisms (SNPs) in MC4R with milk production traits in water buffalo. Eight SNPs were identified by direct polymerase chain reaction sequencing of samples from 18 buffaloes. SNPs were then genotyped using the matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) method in 332 buffaloes.

Generation of bovine (Bos indicus) and buffalo (Bubalus bubalis) adipose tissue derived stem cells: isolation, characterization, and multipotentiality

R. V. Sampaio, Chiaratti, M. R., Santos, D. C. N., Bressan, F. F., Sangalli, J. R., Sá, A. L. A., Silva, T. V. G., Costa, N. N., Cordeiro, M. S., Santos, S. S. D., Ambrósio, C. E., Adona, P. R., Meirelles, F. V., Miranda, M. S., and Ohashi, O. M., “Generation of bovine (Bos indicus) and buffalo (Bubalus bubalis) adipose tissue derived stem cells: isolation, characterization, and multipotentiality”, vol. 14, pp. 53-62, 2015.

Adult stem cells are known for their plasticity and their potential to differentiate into several different cell types; these characteristics have implications for cell therapy and reproductive biotechnologies. In this study, we report on the isolation and characterization of mesenchymal stem cells (MSC) derived from bovine and buffalo adipose tissue. Cells isolated using enzymatic digestion of bovine and buffalo adipose-tissue biopsy samples were grown in vitro for at least 15 passages, verifying their capacity to proliferate.

Water buffalo genome characterization by the Illumina BovineHD BeadChip

R. R. A. Borquis, Baldi, F., de Camargo, G. M. F., Cardoso, D. F., Santos, D. J. A., Lugo, N. H., Sargolzaei, M., Schenkel, F. S., Albuquerque, L. G., and Tonhati, H., “Water buffalo genome characterization by the Illumina BovineHD BeadChip”, vol. 13, pp. 4202-4215, 2014.

To define the best strategies for genomic association studies and genomic selection, it is necessary to determine the extent of linkage disequilibrium (LD) and the genetic structure of the study population. The current study evaluated the transference of genomic information contained in the Illumina BovineHD BeadChip from cattle to buffaloes, and assessed the extent of the LD in buffaloes.

Analysis of SLC11A1 gene expression in healthy water buffalo (Bubalus bubalis) blood cells using qPCR

A. Crisà, De Matteis, G., Scatà, M. C., and Moioli, B., “Analysis of SLC11A1 gene expression in healthy water buffalo (Bubalus bubalis) blood cells using qPCR”, vol. 12, pp. 6957-6967, 2013.

SLC11A1 (solute carrier family 11 member 1 protein) gene influences the initial phase of bacterial cellular infections through macrophage activation. Recent literature on buffalo has attempted to associate the genotype of the polymorphic microsatellite located in the 3ꞌuntranslated region (3ꞌUTR) of the gene, with either susceptibility to brucellosis or with improved macrophage function. Carriers of the (GT)16 allele have been reported to be resistant to brucellosis.

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