Bubalus bubalis

Analysis of DRB3 gene polymorphisms in Jafarabadi, Mediterranean, and Murrah buffaloes from Brazil

N. B. Stafuzza, Olivatto, L. M., Naressi, B. C. M., Tonhati, H., Amaral-Trusty, M. E. J., Stafuzza, N. B., Olivatto, L. M., Naressi, B. C. M., Tonhati, H., Amaral-Trusty, M. E. J., Stafuzza, N. B., Olivatto, L. M., Naressi, B. C. M., Tonhati, H., and Amaral-Trusty, M. E. J., Analysis of DRB3 gene polymorphisms in Jafarabadi, Mediterranean, and Murrah buffaloes from Brazil, vol. 15, p. -, 2016.

The DRB3 gene is an MHC class II gene that has a high degree of polymorphism with more than 100 alleles described in cattle. This variation contributes to differences among individuals in immune responsiveness and disease resistance. In this study, we searched for allelic variants in exon 2 of the DRB3 gene in 80 river buffaloes of three breeds in Brazil using a PCR-RFLP technique. The PCR product showed genetic polymorphism when digested with RsaI, PstI or HaeIII restriction enzymes.

Sequence analysis of the S1PR1 gene in river buffalo

N. B. Stafuzza, Naressi, B. C. M., Borges, M. M., Amaral-Trusty, M. E. J., Stafuzza, N. B., Naressi, B. C. M., Borges, M. M., Amaral-Trusty, M. E. J., Stafuzza, N. B., Naressi, B. C. M., Borges, M. M., and Amaral-Trusty, M. E. J., Sequence analysis of the S1PR1 gene in river buffalo, vol. 15, p. -, 2016.

Recent developments in methodologies for genomic analyses have enabled a significant advance in understanding of the river buffalo genome. The S1PR1 gene has been mapped to buffalo chromosome 6 and bovine chromosome 3; this gene is of interest as it is a candidate for marbling in meat, an important economic trait. Here, we performed next generation sequencing in a buffalo BAC DNA clone and obtained a 54.5-kb sequence encompassing the entire buffalo S1PR1 gene as well as the 27 kb upstream region and the 22 kb downstream region.

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