Bubalus bubalis

A framework radiation hybrid map of buffalo chromosome 1 ordering scaffolds from buffalo genome sequence assembly

N. B. Stafuzza, Naressi, B. C. M., Yang, E., Cai, J. J., and Amaral-Trusty, M. E. J., “A framework radiation hybrid map of buffalo chromosome 1 ordering scaffolds from buffalo genome sequence assembly”, vol. 14, pp. 13096-13104, 2015.

River buffalo chromosome 1 (BBU1) is a sub-metacentric chromosome homologous to bovine chromosomes 1 and 27. In this study, we constructed a new framework radiation hybrid (RH) map from BBU1 using BBURH5000 panel adding nine new genes (ADRB3, ATP2C1, COPB2, CRYGS, P2RY1, SLC5A3, SLC20A2, SST, and ZDHHC2) and one microsatellite (CSSM043) to the set of markers previously mapped on BBU1.

Estimates of genetic parameters for total milk yield over multiple ages in Brazilian Murrah buffaloes using different models

R. C. Sesana, Baldi, F., Borquis, R. R. A., Bignardi, A. B., Hurtado-Lugo, N. A., L. Faro, E., Albuquerque, L. G., and Tonhati, H., “Estimates of genetic parameters for total milk yield over multiple ages in Brazilian Murrah buffaloes using different models”, vol. 13, pp. 2784-2795, 2014.

The objective of this study was to estimate variance components and genetic parameters for accumulated 305-day milk yield (MY305) over multiple ages, from 24 to 120 months of age, applying random regression (RRM), repeatability (REP) and multi-trait (MT) models. A total of 4472 lactation records from 1882 buffaloes of the Murrah breed were utilized. The contemporary group (herd-year-calving season) and number of milkings (two levels) were considered as fixed effects in all models.

Comparative analysis of the river buffalo somatostatin gene

N. B. Stafuzza, Borges, M. M., and Amaral, M. E. J., “Comparative analysis of the river buffalo somatostatin gene”, vol. 13, pp. 10017-10024, 2014.

The somatostatin protein plays a crucial role in the regulation of multiple biological functions, such as growth, fat deposition, and nutrient absorption in vertebrates. Polymorphisms in the somatostatin gene have been associated with growth traits in livestock species, including cattle and goat. In this study, we conducted complete molecular characterization of the somatostatin gene in Bubalus bubalis (Murrah breed) by sequencing a Murrah BAC clone spanning 72,489 base pairs (bp) in length.

Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library

N. B. Stafuzza, Abbey, C. A., Gill, C. A., Womack, J. E., and Amaral, M. E. J., “Construction and preliminary characterization of a river buffalo bacterial artificial chromosome library”, vol. 11, pp. 3013-3019, 2012.

River buffalo genome analyses have advanced significantly in the last decade, and the genome sequence of Bubalus bubalis will be available shortly. Nonetheless, large-insert DNA library resources such as bacterial artificial chromosomes (BAC) are still required for validation and accurate assembly of the genome sequence. We constructed a river buffalo BAC library containing 52,224 clones with an average insert size of 97 kb, representing 1.7 × coverage of the genome.

Cloning and sequencing of the rDNA gene family of the water buffalo (Bubalus bubalis)

C. Y. Pang, Deng, T. X., Tang, D. S., Yang, C. Y., Jiang, H., Yang, B. Z., and Liang, X. W., “Cloning and sequencing of the rDNA gene family of the water buffalo (Bubalus bubalis)”, vol. 11, pp. 2878-2883, 2012.

The rDNA genes coding for ribosomal RNA in animals are complicated repeat sequences with high GC content. We amplified water buffalo rDNA gene sequences with the long and accurate (LA) PCR method, using LA Taq DNA polymerase and GC buffer, based on bioinformatic analysis of related organisms. The rDNA genes were found to consist of 9016 nucleotides, including three rRNA genes and two internal transcribed spacers (ITS), which we named 18S rRNA, ITS1, 5.8S rRNA, ITS2 and 28S rRNA.

Genetic parameter estimates for buffalo milk yield, milk quality and mozzarella production and Bayesian inference analysis of their relationships

R. R. Aspilcueta-Borquis, Di Palo, R., Neto, F. R. Araujo, Baldi, F., de Camargo, G. M. F., de Albuquerque, L. G., Zicarelli, L., and Tonhati, H., “Genetic parameter estimates for buffalo milk yield, milk quality and mozzarella production and Bayesian inference analysis of their relationships”, vol. 9, pp. 1636-1644, 2010.

Buffalo milk has excellent physical and chemical qualities as a consequence of the high percentage of constituents. This milk property is desirable for the dairy industry because it facilitates manufacture of mozzarella cheese. We estimated genetic parameters for milk yield, milk fat and protein and their effects on mozzarella cheese production using Bayesian inference.

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