Brontispa longissima

Transcriptome sequencing and analysis of the coconut leaf beetle, Brontispa longissima

W. Yan, Liu, L., Li, C. X., Huang, S. C., Ma, Z. L., Qin, W. Q., and Peng, Z. Q., “Transcriptome sequencing and analysis of the coconut leaf beetle, Brontispa longissima”, vol. 14, pp. 8359-8365, 2015.

The coconut leaf beetle, Brontispa longissima, is a de­structive pest of palm plants. Although its ecological and biological characteristics are well understood, its genetic information remains largely unknown. To advance our understanding of its molecular ba­sis of biology and ecology, we sequenced and analyzed its whole tran­scriptome by using high-throughput Illumina paired-end sequencing technology. Approximately 8.08 Gb of clean reads were generated in a single run, which were assembled by using Trinity into 41,652 unigenes with an average length of 932 bp.

Isolation and characterization of eight polymorphic microsatellite loci for the coconut pest, Brontispa longissima (Coleoptera: Hispidae)

C. L. Ma, Wu, H. L., Hu, H. Y., Wu, X., Ma, G. C., Fu, Y. G., and Peng, Z. Q., “Isolation and characterization of eight polymorphic microsatellite loci for the coconut pest, Brontispa longissima (Coleoptera: Hispidae)”, vol. 10. pp. 429-432, 2011.

Brontispa longissima is one of the most serious insect pests of coconut in Southeast Asia; it was first discovered on Hainan Island in June 2002. Despite the economic risk associated with this pest, genetic aspects of the invasion process have remained relatively unexplored. Using microsatellite markers, we investigated the population structure, genetic variability and pattern of invasion in various geographic populations. The methodology was based on a modified biotin-capture method. Eight polymorphic microsatellite loci were isolated and characterized for the pest.

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