Glycerol can be used as a substitute for corn for feeding poultry, but there are concerns about how it may affect growth performance and health of the birds. We evaluated the expression of mRNA of growth hormone (GH) and insulin-like growth factor I (IGF-I) in 35-day-old Japanese quails fed different glycerol levels (0, 4, and 8% dietary glycerol instead of corn). Total RNA was extracted from the breast muscle and cDNA was amplified with the use of specific primers for these genes using real-time PCR.
“Expression of growth genes in response to glycerol use in Japanese quail diets”, vol. 11, pp. 3063-3068, 2012.,
“Characterization of myostatin gene (MSTN) of Pekin duck and the association of its polymorphism with breast muscle traits”, vol. 12, pp. 3166-3177, 2013.,
Myostatin, encoded by the MSTN gene, is a negative regulator of muscle growth, and its expression level in muscle tissue is closely correlated with muscle growth and satellite cell proliferation.