Bone defect

Repair of large segmental bone defects in rabbits using BMP and FGF composite xenogeneic bone

X. Li, Lin, Z., Duan, Y., Shu, X., Jin, A., Min, S., and Yi, W., “Repair of large segmental bone defects in rabbits using BMP and FGF composite xenogeneic bone”, vol. 14, pp. 6395-6400, 2015.

The objective of this study was to determine the ability of bone morphogenetic protein (BMP) and fibroblast growth factor (FGF) to repair large segmental radial bone defects in rabbits. We treated calf cancellous bones with 3 mg/L BMP (Group A), 5 μg/L FGF (Group B), or 3 mg/L BMP plus 5 μg/L FGF (Group C). A bone damage model was established using healthy radii from rabbits. The complexes were implanted in the areas of the bone defects in the radii.

Construction of an adenoviral expression vector carrying FLAG and hrGFP-1 genes and its expression in bone marrow mesenchymal stem cells

G. X. Wang, Hu, L., Zhang, Z., and Liu, D. P., “Construction of an adenoviral expression vector carrying FLAG and hrGFP-1 genes and its expression in bone marrow mesenchymal stem cells”, vol. 13, pp. 1070-1078, 2014.

The aim of this study was to construct an adenoviral expression vector for vascular endothelium growth factor 121 (VEGF121)-FLAG and humanized Renilla reniformis green fluorescent protein (hrGFP-1) genes, and to observe their expressions in bone marrow mesenchymal stem cells. Using pTG19T-VEGF121 as a template, polymerase chain reaction technology was adopted to mutate the VEGF121 gene by removing the stop codon and inserting NotI and XhoI restriction sites both before and after the gene sequences.

BMP-2 promotes chondrogenesis of rat adipose-derived stem cells by using a lentiviral system

H. L. Fu, Diao, Z. Y., Shao, L., and Yang, D. P., “BMP-2 promotes chondrogenesis of rat adipose-derived stem cells by using a lentiviral system”, vol. 13, pp. 8620-8631, 2014.

Osteoporosis poses a major public health threat in aging societies. Adipose-derived stem cells (ADSCs) are multipotent adult stem cells that have the ability to yield mesenchymal stem cells, and have the potential to undergo osteogenesis and bone regeneration. Bone morphogenetic proteins (BMPs) have been demonstrated to upregulate bone gene expression after mechanical injury and to improve bone injury repair. This study aimed to produce BMP-2 expression in ADSCs by using lentiviral vectors. Subcutaneous adipose tissue from 4-week-old male Sprague-Dawley rats was used.

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