Black Sigatoka

Virulence and genetic diversity among isolates of Mycosphaerella fijiensis in two regions of Brazil

G. F. Silva, Santos, V. S., Sousa, N. R., Hanada, R. E., Gasparotto, L., Silva, G. F., Santos, V. S., Sousa, N. R., Hanada, R. E., Gasparotto, L., Silva, G. F., Santos, V. S., Sousa, N. R., Hanada, R. E., and Gasparotto, L., Virulence and genetic diversity among isolates of Mycosphaerella fijiensis in two regions of Brazil, vol. 15, p. -, 2016.

Black sigatoka, caused by the fungus Mycosphaerella fijiensis (anamorphic stage: Paracercospora fijiensis), was first detected in Brazil in early 1998 in the Benjamin Constant and Tabatinga municipalities in the State of Amazonas, near to where the borders of Brazil, Colombia, and Peru converge. Understanding how cultivars react to the pathogen, and characterizing the genetic variability of isolates from two distant and distinct banana-producing regions, are important for determining the virulence of M. fijiensis.

Genetic diversity of Mycosphaerella fijiensis in Brazil analyzed using an ERIC-PCR marker

G. F. Silva, Paixão, R. D. V., Queiroz, C. B., Santana, M. F., Souza, A., Sousa, N. R., Hanada, R. E., and Gasparotto, L., Genetic diversity of Mycosphaerella fijiensis in Brazil analyzed using an ERIC-PCR marker, vol. 13, pp. 7698-7707, 2014.

The Enterobacterial repetitive intergenic consensus (ERIC) marker was used to analyze the genetic variability of Mycosphaerella fijiensis, the causative agent of Black Sigatoka disease in banana plants. A total of 123 isolates were used, which were divided into populations based on their original hosts and collection sites in Brazil. A total of 9 loci were amplified, 77.8% of which were found to be polymorphic. The genetic diversity found in the population was 0.20.

Variable number of tandem repeat markers in the genome sequence of Mycosphaerella fijiensis, the causal agent of black leaf streak disease of banana (Musa spp)

S. A. L. Garcia, Van der Lee, T. A. J., Ferreira, C. F., B. Hekkert, T. Lintel, Zapater, M. - F., Goodwin, S. B., Guzmán, M., Kema, G. H. J., and Souza, Jr., M. T., Variable number of tandem repeat markers in the genome sequence of Mycosphaerella fijiensis, the causal agent of black leaf streak disease of banana (Musa spp), vol. 9, pp. 2207-2212, 2010.

We searched the genome of Mycosphaerella fijiensis for molecular markers that would allow population genetics analysis of this plant pathogen. M. fijiensis, the causal agent of banana leaf streak disease, also known as black Sigatoka, is the most devastating pathogen attacking bananas (Musa spp). Recently, the entire genome sequence of M. fijiensis became available. We screened this database for VNTR markers. Forty-two primer pairs were selected for validation, based on repeat type and length and the number of repeat units.

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