bla gene

Genetic markers for detection of Escherichia coli K-12 harboring ampicillin-resistance plasmid from an industrial wastewater treatment effluent pond

G. A. R. Simões, Xavier, M. A. S., Oliveira, D. A., Menezes, E. V., Magalhães, S. S. G., Gandra, J. A. C. D., Xavier, A. R. E. O., Simões, G. A. R., Xavier, M. A. S., Oliveira, D. A., Menezes, E. V., Magalhães, S. S. G., Gandra, J. A. C. D., and Xavier, A. R. E. O., Genetic markers for detection of Escherichia coli K-12 harboring ampicillin-resistance plasmid from an industrial wastewater treatment effluent pond, vol. 15, p. -, 2016.

Biotechnology industries that use recombinant DNA technology are potential sources for release of genetically modified organisms to the environment. Antibiotic-resistance marker genes are commonly used for recombinant bacteria selection. One example is the marker gene coding for β-lactamase (bla) in plasmids found in Escherichia coli K-12. The aim of this study was to provide an approach to develop a molecular method for genetic marker detection in E. coli K-12 harboring bla genes from an industrial wastewater treatment effluent pond (IWTEP).

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