Biological activity

Expression of recombinant myostatin propeptide pPIC9K-Msp plasmid in Pichia pastoris

W. Du, Xia, J., Zhang, Y., Liu, M. J., Li, H. B., Yan, X. M., Zhang, J. S., Li, N., Zhou, Z. Y., and Xie, W. Z., Expression of recombinant myostatin propeptide pPIC9K-Msp plasmid in Pichia pastoris, vol. 14, pp. 18414-18420, 2015.

Myostatin propeptide can inhibit the biological activity of myostatin protein and promote muscle growth. To express myostatin propeptide in vitro with a higher biological activity, we performed codon optimization on the sheep myostatin propeptide gene sequence, and mutated aspartic acid-76 to alanine based on the codon usage bias of Pichia pastoris and the enhanced biological activity of myostatin propeptide mutant. Modified myostatin propeptide gene was cloned into the pPIC9K plasmid to form the recombinant plasmid pPIC9K-Msp.

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