Biodegradation

Diversity of propanil-degrading bacteria isolated from rice rhizosphere and their potential for plant growth promotion

A. R. L. Procópio, Procópio, R. E. L., Pizzirani-Kleiner, A. A., and Melo, I. S., Diversity of propanil-degrading bacteria isolated from rice rhizosphere and their potential for plant growth promotion, vol. 11, pp. 2021-2034, 2012.

The herbicide propanil has long been used in rice production in southern Brazil. Bacteria isolated from contaminated soils in Massaranduba, Santa Catarina, Brazil, were found to be able to grow in the presence of propanil, using this compound as a carbon source. Thirty strains were identified as Pseudomonas (86.7%), Serratia (10.0%), and Acinetobacter (3.3%), based on phylogenetic analysis of 16S rDNA.

Construction and validation of metagenomic DNA libraries from landfarm soil microorganisms

T. B. A. Pessoa, de Souza, S. S., Cerqueira, A. F., Rezende, R. P., Pirovani, C. P., and Dias, J. C. T., Construction and validation of metagenomic DNA libraries from landfarm soil microorganisms, vol. 12, pp. 2148-2155, 2013.

Landfarming biodegradation is a strategy used by the petrochemical industry to reduce pollutants in petroleum-contaminated soil. We constructed 2 metagenomic libraries from landfarming soil in order to determine the pathway used for mineralization of benzene and to examine protein expression of the bacteria in these soils. The DNA of landfarm soil, collected from Ilhéus, BA, Brazil, was extracted and a metagenomic library was constructed with the Copy ControlTM Fosmid Library Production Kit, which clones 25-45-kb DNA fragments.

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