Benzene

Construction and validation of metagenomic DNA libraries from landfarm soil microorganisms

T. B. A. Pessoa, de Souza, S. S., Cerqueira, A. F., Rezende, R. P., Pirovani, C. P., and Dias, J. C. T., “Construction and validation of metagenomic DNA libraries from landfarm soil microorganisms”, vol. 12, pp. 2148-2155, 2013.

Landfarming biodegradation is a strategy used by the petrochemical industry to reduce pollutants in petroleum-contaminated soil. We constructed 2 metagenomic libraries from landfarming soil in order to determine the pathway used for mineralization of benzene and to examine protein expression of the bacteria in these soils. The DNA of landfarm soil, collected from Ilhéus, BA, Brazil, was extracted and a metagenomic library was constructed with the Copy ControlTM Fosmid Library Production Kit, which clones 25-45-kb DNA fragments.

Expression of multidrug resistance 1 and multidrug resistance-related protein 1 in C57BL/6 mice treated with benzene

J. - S. Huang, Zhao, M. - D., Shi, J. - M., Zhang, J. - H., Li, B., Fan, W., and Zhou, Y. - L., “Expression of multidrug resistance 1 and multidrug resistance-related protein 1 in C57BL/6 mice treated with benzene”, vol. 12, pp. 5842-5850, 2013.

ATP-binding cassette super family (ABC) proteins are considered key to oncology and pharmacology studies. We examined the effect of benzene on ABC pump protein levels in C57BL/6 mouse bone marrow mononuclear cells. After a 2-week gavage (200 mg/kg, 5 days per week), the number of peripheral leukocytes, lymphocytes and basophils dropped significantly; there was also a significant decrease in MDR1 and MRP1 gene expression. A significant reduction in expression of P-gp was found; however, there was no significant decrease in the expression of MRP1 and NF-κB p65.

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