Bama mini-pig

Isolation, proliferation, and induction of Bama mini-pig spermatogonial stem cells in vitro

H. M. Zhao, Yang, H., Luo, F. H., Li, M. X., Zhang, S., Yang, X. G., Lu, Y. Q., Lu, S. S., Wu, Y. J., Lu, K. H., Zhao, H. M., Yang, H., Luo, F. H., Li, M. X., Zhang, S., Yang, X. G., Lu, Y. Q., Lu, S. S., Wu, Y. J., and Lu, K. H., Isolation, proliferation, and induction of Bama mini-pig spermatogonial stem cells in vitro, vol. 15, p. -, 2016.

Spermatogonial stem cells (SSCs), the unique seed cells of testes, can undergo meiosis and form spermatozoa, thus transmitting genetic information to offspring. Research concerning these cells explores the mechanism underlying spermatogenesis, making possible the induction of their differentiation into spermatozoa in vitro. SSCs have therefore attracted much interest among scientists. Although the proliferation of such cells in vitro has been demonstrated, we are unaware of any long-term laboratory culture of porcine SSCs.

Production of hGFAP-DsRed transgenic Guangxi Bama mini-pigs via somatic cell nuclear transfer

L. L. Hu, Lu, Y. Q., Xu, H. Y., Yang, X. G., Lu, S. S., and Lu, K. H., Production of hGFAP-DsRed transgenic Guangxi Bama mini-pigs via somatic cell nuclear transfer, vol. 14, pp. 16285-16296, 2015.

The mini-pig is a useful animal model for human biomedical research due to its physiological similarity to humans and the ease of handling. In order to optimize the efficiency of production of transgenic Bama mini-pigs through somatic cell nuclear transfer (SCNT), we examined the effects of contact inhibition, roscovitine treatment, and serum starvation on the cell cycle synchronization and transgenic cloned embryo development in vivo and in vitro after nuclear transfer.

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