Bacteria

Predicting bacterial essential genes using only sequence composition information

L. W. Ning, Lin, H., Ding, H., Huang, J., Rao, N., and Guo, F. B., Predicting bacterial essential genes using only sequence composition information, vol. 13, pp. 4564-4572, 2014.

Essential genes are those genes that are needed by organisms at any time and under any conditions. It is very important for us to identify essential genes from bacterial genomes because of their vital role in synthetic biology and biomedical practices. In this paper, we developed a support vector machine (SVM)-based method to predict essential genes of bacterial genomes using only compositional features. These features are all derived from the primary sequences, i.e., nucleotide sequences and protein sequences.

Drug resistance analysis of bacterial strains isolated from burn patients

L. F. Wang, Li, J. L., Ma, W. H., and Li, J. Y., Drug resistance analysis of bacterial strains isolated from burn patients, vol. 13, pp. 9727-9734, 2014.

This study aimed to analyze the spectrum and drug resistance of bacteria isolated from burn patients to provide a reference for rational clinical use of antibiotics. Up to 1914 bacterial strain specimens isolated from burn patients admitted to hospital between 2001 and 2010 were subjected to resistance monitoring by using the K-B paper disk method. Retrospective analysis was performed on drug resistance analysis of burn patients. The top eight bacterium strains according to detection rate.

Lignocellulolytic enzymes and bacteria associated with the digestive tracts of Stenochironomus (Diptera: Chironomidae) larvae

R. Koroiva, Souza, C. W. O., Toyama, D., Henrique-Silva, F., and Fonseca-Gessner, A. A., Lignocellulolytic enzymes and bacteria associated with the digestive tracts of Stenochironomus (Diptera: Chironomidae) larvae, vol. 12, pp. 3421-3434, 2013.

We analyzed the digestive activity of the enzymes that digest cellulose and hemicellulose and the bacterial community that is capable of hydrolyzing wood compounds in the digestive tracts of Stenochironomus (Diptera: Chironomidae) larvae, which are miners of decomposing submerged tree and bush branches. Based on quantification of reducing sugars, these larvae have a limited capacity for cellulose degradation but a good capacity for xylan hydrolysis.

Antigenotoxic, and anticytotoxic activities of an ethanolic extract of Lafoensia pacari (Lythraceae) stem bark in bacteria and mice

D. C. S. Lima, Silva, C. R., Sampaio, B. L., de Paula, J. R., and Chen-Chen, L., Antigenotoxic, and anticytotoxic activities of an ethanolic extract of Lafoensia pacari (Lythraceae) stem bark in bacteria and mice, vol. 12, pp. 3887-3896, 2013.

Lafoensia pacari (Lythraceae), popularly known in Brazil as "pacari", is a small tree native to the Cerrado that is used in folk medicine to treat cancer and as an anti-inflammatory and cicatrizing agent. We evaluated the genotoxic, cytotoxic, antigenotoxic, and anticytotoxic activities of an ethanol extract of L. pacari stem bark (EESB) using the Ames test and the mouse bone marrow micronucleus test.

Application of RNA-seq to reveal the transcript profile in bacteria

A. C. Pinto, Melo-Barbosa, H. P., Miyoshi, A., Silva, A., and Azevedo, V., Application of RNA-seq to reveal the transcript profile in bacteria, vol. 10. pp. 1707-1718, 2011.

The large number of microbial genomes deposited in databanks has opened the door for in-depth studies of organisms, including post-genomics investigations. Thanks to new generation sequencing technology, these studies have made advances that have lead to extraordinary discoveries in bacterial transcriptomics. In this review, we describe bacterial RNA sequencing studies that use these new techniques.

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