B cell

Impact of caffeine on β cell proliferation and apoptosis under the influence of palmitic acid

L. Chen, Yu, M., Shen, T., Xia, J., and Xu, B. L., “Impact of caffeine on β cell proliferation and apoptosis under the influence of palmitic acid”, vol. 14, pp. 5724-5730, 2015.

We examined the influence of caffeine on the proliferation and apoptosis of β cells cultured in vitro in the presence of the free fatty acid palmitic acid (PA). Different concentrations of caffeine (1-100 mM) and free fatty PA were added to cultured β cells. The MTT assay was used to analyze cell proliferative activity; flow cytometry was used to measure apoptosis and calculate the apoptosis rate. Compared with the blank control group, cells cultured with 500 mM PA for 24, 48, 72, and 96 h showed inhibition of pancreatic β cell proliferative activity.

Screening and functional microarray analysis of differentially expressed genes related to osteoporosis

Y. Chen and Xia, R. G., “Screening and functional microarray analysis of differentially expressed genes related to osteoporosis”, vol. 13, pp. 3228-3236, 2014.

We searched for key genes that could accurately predict bone mineral density. The gene expression profile GSE7429 was downloaded from the Gene Expression Omnibus database, which includes 20 samples, 10 with high and 10 with low bone mineral density. The differentially expressed genes (DEGs) were identified with packages in R language. Further, BLASTX was used to obtain COG function classifications of all the DEGs. The GOTM software was used to find DEGs enriched modules. The functions of genes in the modules was also predicted with the software GENECODIS.

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