AtExpA8

Efficient use of artificial micro-RNA to downregulate the expression of genes at the post-transcriptional level in Arabidopsis thaliana

A. Ud-Din, Rauf, M., Ghafoor, S., Khattak, M. N. K., Hameed, M. W., Shah, H., Jan, S., Muhammad, K., Rehman, A., Inamullah,, Ud-Din, A., Rauf, M., Ghafoor, S., Khattak, M. N. K., Hameed, M. W., Shah, H., Jan, S., Muhammad, K., Rehman, A., Inamullah,, Ud-Din, A., Rauf, M., Ghafoor, S., Khattak, M. N. K., Hameed, M. W., Shah, H., Jan, S., Muhammad, K., Rehman, A., and Inamullah, Efficient use of artificial micro-RNA to downregulate the expression of genes at the post-transcriptional level in Arabidopsis thaliana, vol. 15, p. -, 2016.

Micro-RNAs are cellular components regulating gene expression at the post-transcription level. In the present study, artificial micro-RNAs were used to decrease the transcript level of two genes, AtExpA8 (encoding an expansin) and AHL25 (encoding an AT-hook motif nuclear localized protein) in Arabidopsis thaliana. The backbone of the Arabidopsis endogenous MIR319a micro-RNA was used in a site-directed mutagenesis approach for the generation of artificial micro-RNAs targeting two genes.

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