ARMS-PCR

Development of a new amplification-refractory mutation system for detection of a single nucleotide polymorphism linked to drug resistance in Ancylostoma caninum

L. F. Furtado and Rabelo, É. M., Development of a new amplification-refractory mutation system for detection of a single nucleotide polymorphism linked to drug resistance in Ancylostoma caninum, vol. 14, pp. 5103-5111, 2015.

Single nucleotide polymorphisms at codons 167, 198, and 200 in the β-tubulin isotype 1 gene have been associated with benz­imidazole resistance. Until now, the only mutation observed in Ancy­lostoma caninum was at codon 200 of this gene. However, the standard­ized methodologies used to detect mutations in this species are faulty. The objective of this study was to standardize a molecular technique based on amplification-refractory mutation system-polymerase chain reaction (ARMS-PCR) for detecting the mutation at codon 200 in the A.

Ovine prion protein genotype frequencies in northwestern China

C. - L. Zhao, Wu, R., Liu, L., Li, F. - D., Zhang, X. - L., Wang, C., Wang, F., Diao, X. - L., Guan, H. - W., Wang, X., and Zhou, L., Ovine prion protein genotype frequencies in northwestern China, vol. 11, pp. 1671-1681, 2012.

Anti-scrapie breeding programs have been initiated to screen for scrapie-resistant sheep based on ovine prion protein gene (PRNP) genotypes at codons 136, 154 and 171 in many countries, especially European Union member states. However, investigation of sheep PRNP genotypes is limited in China, despite the large number of sheep breeds. We analyzed 432 sheep of five different breeds from farms in northwestern China, using PCR-single-strand conformational polymorphism analysis (PCR-SSCP); the corresponding haplotypes of different PRNP alleles were cloned.

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