Arabidopsis thaliana

Efficient use of artificial micro-RNA to downregulate the expression of genes at the post-transcriptional level in Arabidopsis thaliana

A. Ud-Din, Rauf, M., Ghafoor, S., Khattak, M. N. K., Hameed, M. W., Shah, H., Jan, S., Muhammad, K., Rehman, A., Inamullah,, Ud-Din, A., Rauf, M., Ghafoor, S., Khattak, M. N. K., Hameed, M. W., Shah, H., Jan, S., Muhammad, K., Rehman, A., Inamullah,, Ud-Din, A., Rauf, M., Ghafoor, S., Khattak, M. N. K., Hameed, M. W., Shah, H., Jan, S., Muhammad, K., Rehman, A., and Inamullah, Efficient use of artificial micro-RNA to downregulate the expression of genes at the post-transcriptional level in Arabidopsis thaliana, vol. 15, p. -, 2016.

Micro-RNAs are cellular components regulating gene expression at the post-transcription level. In the present study, artificial micro-RNAs were used to decrease the transcript level of two genes, AtExpA8 (encoding an expansin) and AHL25 (encoding an AT-hook motif nuclear localized protein) in Arabidopsis thaliana. The backbone of the Arabidopsis endogenous MIR319a micro-RNA was used in a site-directed mutagenesis approach for the generation of artificial micro-RNAs targeting two genes.

Protein expression and characterization of SEP3 from Arabidopsis thaliana

Q. Shi, Zhou, J., Wang, P., Lin, X., and Xu, Y., Protein expression and characterization of SEP3 from Arabidopsis thaliana, vol. 14, pp. 12529-12536, 2015.

SEPALLATA (SEP) MADS-box genes play crucial roles in the regulation of floral growth and development. They are required for the specification of sepals, petals, stamens, and carpels as well as for floral determinacy. SEPs perform their functions through the formation of homo- or hetero-polymers, which are the molecular basis of floral quartets. In vitro assays indicated that SEP3 forms a tetramer after binding to DNA, but it is unclear whether DNA binding induces the tetramer, because SEP3 is often reported to form a dimer.

Isolation of Pantoea ananatis from sugarcane and characterization of its potential for plant growth promotion

J. F. da Silva, Barbosa, R. R., de Souza, A. N., da Motta, O. V., Teixeira, G. N., Carvalho, V. S., de Souza, A. L. S. R., and Filho, G. Ade Souza, Isolation of Pantoea ananatis from sugarcane and characterization of its potential for plant growth promotion, vol. 14, pp. 15301-15311, 2015.

Each year, approximately 170 million metric tons of chemical fertilizer are consumed by global agriculture. Furthermore, some chemical fertilizers contain toxic by-products and their long-term use may contaminate groundwater, lakes, and rivers. The use of plant growth-promoting bacteria may be a cost-effective strategy for partially replacing conventional chemical fertilizers, and may become an integrated plant nutrient solution for sustainable crop production.

Molecular cloning and characterization of a subtilisin-like protease from Arabidopsis thaliana

D. H. Li, Xi, H., Yu, X. B., and Cai, Y. P., Molecular cloning and characterization of a subtilisin-like protease from Arabidopsis thaliana, vol. 14, pp. 16535-16545, 2015.

The Arabidopsis thaliana genome encodes 56 subtilisin-like serine proteases (subtilases). In order to evaluate the protease activity of a previously uncharacterized subtilase, designated as AtSBT1.9, we cloned its full-length cDNA from A. thaliana seedlings. An AtSBT1.9 mature peptide coding sequence was inserted into the bacterial expression vector, pMAL-c2x, and the recombinant vector was transformed into Escherichia coli BL21 (DE3).

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