Arabidopsis thaliana

Expression of recombinant human anti-TNF-α scFv-Fc in Arabidopsis thaliana seeds

N. Yao, Ai, L., Dong, Y. Y., Liu, X. M., Wang, D. Z., Wang, N., Li, X. W., Wang, F. W., Li, X. K., Li, H. Y., Jiang, C., Yao, N., Ai, L., Dong, Y. Y., Liu, X. M., Wang, D. Z., Wang, N., Li, X. W., Wang, F. W., Li, X. K., Li, H. Y., and Jiang, C., Expression of recombinant human anti-TNF-α scFv-Fc in Arabidopsis thaliana seeds, vol. 15, p. -, 2016.

Recombinant human anti-tumor necrosis factor (TNF)-α scFv-Fc was expressed in TKO mutant Arabidopsis thaliana seeds using plant-specific codons. Immunoblotting using a human IgG1 antibody detected the expression of anti-TNF-α proteins in plants. Results from qRT-PCR analysis demonstrated that the time of harvest significantly affected the protein yield and quality. Our results indicate that the Phaseolus vulgaris β-phaseolin promoter directed anti-TNF-α scFv-Fc expression in A. thaliana seeds, with a maximum yield obtained at 20-days of development.

Efficient use of artificial micro-RNA to downregulate the expression of genes at the post-transcriptional level in Arabidopsis thaliana

A. Ud-Din, Rauf, M., Ghafoor, S., Khattak, M. N. K., Hameed, M. W., Shah, H., Jan, S., Muhammad, K., Rehman, A., Inamullah,, Ud-Din, A., Rauf, M., Ghafoor, S., Khattak, M. N. K., Hameed, M. W., Shah, H., Jan, S., Muhammad, K., Rehman, A., Inamullah,, Ud-Din, A., Rauf, M., Ghafoor, S., Khattak, M. N. K., Hameed, M. W., Shah, H., Jan, S., Muhammad, K., Rehman, A., and Inamullah, Efficient use of artificial micro-RNA to downregulate the expression of genes at the post-transcriptional level in Arabidopsis thaliana, vol. 15, p. -, 2016.

Micro-RNAs are cellular components regulating gene expression at the post-transcription level. In the present study, artificial micro-RNAs were used to decrease the transcript level of two genes, AtExpA8 (encoding an expansin) and AHL25 (encoding an AT-hook motif nuclear localized protein) in Arabidopsis thaliana. The backbone of the Arabidopsis endogenous MIR319a micro-RNA was used in a site-directed mutagenesis approach for the generation of artificial micro-RNAs targeting two genes.

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