Aquaculture
Morphology and muscle gene expression in GIFT and Supreme Nile tilapia varieties reared in two cultivation systems.
Morphology and muscle gene expression in GIFT and Supreme Nile tilapia varieties reared in two cultivation systems.
Effect of propolis ethanol extract on myostatin gene expression and muscle morphometry of Nile tilapia in net cages.
Genetic diversity and genetic structure of consecutive breeding generations of golden mandarin fish (Siniperca scherzeri Steindachner) using microsatellite markers
In this study, 12 polymorphic microsatellites were investigated to determine the genetic diversity and structure of 5 consecutive selected populations of golden mandarin fish (Siniperca scherzeri Steindachner). The total numbers of alleles, average heterozyosity, and average polymorphism information content showed that the genetic diversity of these breeding populations was decreasing.
New microsatellite markers for the abalone Haliotis midae developed by 454 pyrosequencing and in silico analyses
Farming of Haliotis midae is the most lucrative aquaculture venture in South Africa. The genome of this species needs to be studied to assist in selective breeding programs aimed at increasing overall yield, and molecular markers will be required to attain this goal. We identified and characterized 82 polymorphic microsatellite loci by using repeat-enriched genomic libraries and high-throughput pyrosequencing technology.
Development and characterization of 60 microsatellite markers in the abalone Haliotis diversicolor
The abalone, Haliotis diversicolor, is one of the most important mariculture species in southern China. We developed 60 new polymorphic microsatellite markers for H. diversicolor and characterized them in 30 individuals from a cultured population in Sanya, China. All 60 markers were found to be polymorphic. The number of alleles ranged from two to nine per locus, with an average of 4.12/locus. The expected and observed heterozygosities ranged from 0.10 to 0.88 and from 0.07 to 0.87, respectively. Forty-four loci were in Hardy-Weinberg equilibrium.
Eight polymorphic microsatellite markers for the spotted babylon, Babylonia areolata (Buccinidae)
The spotted babylon, Babylonia areolata, is one of the most extensively cultured marine mollusks in southeast Asia. Eight polymorphic microsatellite markers were developed for this species, from a microsatellite-enriched library. These markers, characterized in 32 individuals from a hatchery population, were polymorphic, with allele numbers ranging from 6 to 18 per locus, expected and observed heterozygosities ranging from 0.68 to 0.94 and 0.56 to 0.81, respectively.