Apoptosis

Effects of cetuximab combined with afatinib on the expression of KDR and AQP1 in lung cancer

Y. H. Liu and Zhu, W. L., “Effects of cetuximab combined with afatinib on the expression of KDR and AQP1 in lung cancer”, vol. 14, pp. 16652-16661, 2015.

In this study, we examined the effect of cetuximab (epidermal growth factor receptor monoclonal antibody) combined with afatinib (epidermal growth factor receptorand human epidermal growth factor receptor 2 tyrosine kinase irreversible inhibitor) on the apoptosis of A549 cells and on kinase domain receptor (KDR) and aquaporin 1 (AQP1) expression in A549 cells. A549 cells were cultured in RPMI-1640 and then divided into 4 groups: control group, 1-nM cetuximab group, 25-μM afatinib group, and 1-nM cetuximab + 25-μM afatinib group.

Expression and effect of serum interleukin-24 level on bone marrow mononuclear cells in children with acute leukemia

H. R. Cheng, Wu, B. Q., Chen, L., Zhang, Z. X., and Li, B., “Expression and effect of serum interleukin-24 level on bone marrow mononuclear cells in children with acute leukemia”, vol. 14, pp. 17281-17288, 2015.

To investigate the expression of interleukin-24 (IL-24) in the children with acute leukemia (AL) and its effect on the apoptosis of bone marrow mononuclear cells (BMMNCs) in vitro. Four groups were assessed: acute lymphocytic leukemia (ALL), acute myeloid leukemia (AML), non-leukemia, and healthy groups, 20 children in each group. ELISA was used to measure IL-24 serum level. The bone marrow was taken from patients and controls. BMMNCs were isolated and the DNA was analyzed by glucose electrophoresis. Flow cytometry was used to determine BMMNC apoptosis.

O-naphthoquinone isolated from Capraria biflora L. induces selective cytotoxicity in tumor cell lines

G. G. Nde S. Wisintainer, Scola, G., Moura, S., Lemos, T. L. G., Pessoa, C., de Moraes, M. O., Souza, L. G. S., Roesch-Ely, M., and Henriques, J. A. P., “O-naphthoquinone isolated from Capraria biflora L. induces selective cytotoxicity in tumor cell lines”, vol. 14, pp. 17472-17481, 2015.

Biflorin is an o-naphthoquinone isolated from the roots of the plant Capraria biflora L. (Scrophulariaceae). In this study, the cytotoxic effects of biflorin were verified, and late apoptosis was detected in various cancer cell lines by in situ analysis. The cytotoxicity was further evaluated exclusively for 48 h of treatment in different tumor and non-tumor cell lines (Hep-2, HeLa, HT-29, A-375, and A-549, and HEK-293, respectively). The results indicated that biflorin induced selective cytotoxicity in tumor cells.

Aquaporin in the proliferation and apoptosis of diabetic myocardial cells

P. F. Zhao and Sun, M. J., “Aquaporin in the proliferation and apoptosis of diabetic myocardial cells”, vol. 14, pp. 17366-17372, 2015.

The aim of this study was to explore the effect of aquaporin on the molecular mechanism of human diabetic myocardial cell apoptosis. The methylthiazolyle tetrazolium assay was used to detect the inhibitory effect of different concentrations of aquaporin on cell growth. The rate of aquaporin-induced myocardial cell apoptosis was detected by flow cytometric analysis of Annexin V-fluorescein isothiocyanate/propidium iodide double-stained cells. We also attempted to quantify the expression of Bcl-2, Bax, caspase-3, and survivin in diabetic myocardial cells by western blot analysis.

Sevoflurane induces neurotoxicity in young mice through FAS/FASL signaling

Q. Song, Ma, Y. L., Song, J. Q., Chen, Q., Xia, G. S., Ma, J. Y., Feng, F., Fei, X. J., and Wang, Q. M., “Sevoflurane induces neurotoxicity in young mice through FAS/FASL signaling”, vol. 14, pp. 18059-18068, 2015.

Sevoflurane, the most widely used anesthetic in clinical practice, has been shown to induce apoptosis, inhibit neurogenesis, and cause learning and memory impairment in young mice. However, the underlying mechanism is still unknown. In this study, wild-type and the FAS- or FAS ligand (FASL)-knockout mice (age 7 days) were exposed to sevoflurane or pure oxygen. Western blotting was used to examine the expression of FAS protein.

Effect and mechanism of miR-126 in myocardial ischemia reperfusion

B. Li, Tao, Y., and Huang, Q., “Effect and mechanism of miR-126 in myocardial ischemia reperfusion”, vol. 14, pp. 18990-18998, 2015.

Multiple studies have shown microRNAs to play an important role in disease occurrence and development. The role of miRNAs in ischemia-reperfusion injury, however, requires further investigation and the aim of this study was therefore to assess miR-126 expression in myocardial ischemia reperfusion and the effects of miR-126 on myocardial ischemia-reperfusion injury. An in vitro model of ischemia-reperfusion injury was established using rat myocardial H9c2 cells and miR-126 expression in these cells was assessed by real-time PCR.

Forkhead box protein O1 mediates apoptosis in a cancer cervical cell line treated with the antitumor agent tumor necrosis factor-α

B. Zhang, Gui, L., Zhu, L., Zhao, X., Yang, Y., and Li, Q., “Forkhead box protein O1 mediates apoptosis in a cancer cervical cell line treated with the antitumor agent tumor necrosis factor-α”, vol. 14, pp. 7446-7454, 2015.

Tumor necrosis factor-α (TNF-α) is an important pro-apoptotic cytokine, which performs a broad range of immune and inflammatory functions in several vital processes. TNF-α-induced apoptosis has been confirmed, however, relatively little is known regarding the role of forkhead box class-O 1 (FOXO1) in mediating TNF-α-induced apoptosis in cervical cancer. In our study, we used the well-characterized cervical cancer cell line C-33A to investigate the role of FOXO1.

Neuroprotective effects of Bcl-2 overexpression on nerve cells of rats with acute cerebral infarction

H. R. Zhang, Peng, J. H., Zhu, G. Y., and Xu, R. X., “Neuroprotective effects of Bcl-2 overexpression on nerve cells of rats with acute cerebral infarction”, vol. 14, pp. 7696-7703, 2015.

We aimed to investigate the influence of lentiviral-medi­ated Bcl-2 overexpression in cerebral tissues of rats with acute cerebral infarction. Forty-five rats were randomly divided into sham, model, and treatment groups. The sham and model groups were administered a control lentiviral vector via the intracranial arteries 10 days before surgery, while the treatment group received lentivirus encoding a Bcl-2 overexpression vector.

Expression and localization of cFLIP anti-apoptotic protein in the porcine corpus luteum and corpora albicans during the estrous cycle and pregnancy

H. Z. Jin, Shi, W. S., Tian, Y., Liu, Y., Jin, Y., and Manabe, N., “Expression and localization of cFLIP anti-apoptotic protein in the porcine corpus luteum and corpora albicans during the estrous cycle and pregnancy”, vol. 14, pp. 8262-8272, 2015.

We determined expression and localization of the anti-apoptotic cellular FLICE inhibitory protein (cFLIP) in the porcine corpora lutea (CL) and corpus albicans (CA) during estrous and preg­nancy. The CL and CA were collected at different stages of estrous to determine cFLIP immunolocalization, and mRNA and protein ex­pression.

Mechanism of rat osteosarcoma cell apoptosis induced by a combination of low-intensity ultrasound and 5-aminolevulinic acid in vitro

Y. N. Li, Zhou, Q., Yang, B., Hu, Z., Wang, J. H., Li, Q. S., and Cao, W. W., “Mechanism of rat osteosarcoma cell apoptosis induced by a combination of low-intensity ultrasound and 5-aminolevulinic acid in vitro”, vol. 14, pp. 9604-9613, 2015.

We investigated the killing effect of low-intensity ultrasound combined with 5-aminolevulinic acid (5-ALA) on the rat osteosarcoma cell line UMR-106. Logarithmic-phase UMR-106 cells were divided into a control group, ultrasound group and 5-ALA group. The cell apoptotic rate, production of reactive oxygen species, and the change in mitochondrial membrane potential were analyzed by flow cytometry; ultrastructural changes were observed by transmission electron microscopy.

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