Apoptosis

Docetaxel enhances apoptosis and G2/M cell cycle arrest by suppressing mitogen-activated protein kinase signaling in human renal clear cell carcinoma

T. D. Han, Shang, D. H., Tian, Y., Han, T. D., Shang, D. H., and Tian, Y., Docetaxel enhances apoptosis and G2/M cell cycle arrest by suppressing mitogen-activated protein kinase signaling in human renal clear cell carcinoma, vol. 15, p. -, 2016.

Tremendous efforts have been made in renal cell carcinoma (RCC) patients’ research; however, clinical findings in patients have been disappointing. The aims of our study were to identify better or alternative therapeutic methods that can reverse chemotherapy resistance and to enhance sensitivity to docetaxel (DOX)-based chemotherapy drugs. We evaluated the anti-proliferative effect of DOX against RCC cells. DOX was found to suppress proliferation of RCC cells under in vitro and in vivo settings.

Changes in cFLIP expression in the corpus luteum throughout the estrous cycle of Shibagoats

H. Z. Jin, Wang, Y., Liu, Y., Li, X. P., and Jin, Y., Changes in cFLIP expression in the corpus luteum throughout the estrous cycle of Shibagoats, vol. 14, pp. 12955-12966, 2015.

Changes in the expression of the anti-apoptotic protein cFLIP (cellular FLICE inhibitory protein) were examined in the caprine corpus luteum (CL), during development and subsequent maintenance. Corpora lutea at four different stages were collected from Shiba goats, to measure the expression of cFLIP mRNA, protein and immunolocalization. Expression of short form cFLIP (cFLIPS) mRNA was highest at the early CL stage, and decreased during late and regressed stages (P

Propofol induces apoptosis and inhibits the proliferation of rat embryonic neural stem cells via gamma-aminobutyric acid type A receptor

J. W. Wang, Cheng, W. W., Xu, T., and Yang, Z. Y., Propofol induces apoptosis and inhibits the proliferation of rat embryonic neural stem cells via gamma-aminobutyric acid type A receptor, vol. 14, pp. 14920-14928, 2015.

We investigated the effect of propofol on the proliferation and viability of rat embryonic neural stem cells (rENSCs) and the potential mechanisms involved. rENSCs were isolated and cultured in vitro and treated with 1, 10, or 50 μM propofol, while the control group was treated with 0.1 μM dimethyl sulfoxide. The effect of propofol on the proliferation and viability of rENSCs was examined by proliferation and apoptosis assays. Real-time polymerase chain reaction was employed to analyze the mRNA expression of checkpoint kinase 1 (Chk1) and p53 in rENSCs exposed to propofol.

Effect of wilfortrine on human hepatic cancer HepG2 cell proliferation potential in vitro

M. Yue, Shen, X. J., Liu, Y. X., Lin, X. Y., Zhou, S. Q., and Song, Y. H., Effect of wilfortrine on human hepatic cancer HepG2 cell proliferation potential in vitro, vol. 14, pp. 15349-15355, 2015.

Liver cancers are characterized by high morbidity and mortality owing to few effective drugs for its treatment. Wilfortrine has several pharmacological effects, including an inhibitory effect on liver cancer cell proliferation. However, whether wilfortrine can induce liver cancer cell apoptosis has not been elucidated. We investigated the role of wilfortrine on liver cancer cell HepG2 apoptosis and analyzed its possible mechanisms to provide a theoretical basis for clinical analysis of liver cancer pathogenesis. The liver cancer cell line HepG2 was treated with 40 mM wilfortrine for 48 h.

Dihydromyricetin induces cell apoptosis via a p53-related pathway in AGS human gastric cancer cells

F. J. Ji, Tian, X. F., Liu, X. W., Fu, L. B., Wu, Y. Y., Fang, X. D., and Jin, H. Y., Dihydromyricetin induces cell apoptosis via a p53-related pathway in AGS human gastric cancer cells, vol. 14, pp. 15564-15571, 2015.

The aim of the present study was to determine the anti­proliferative and pro-apoptotic effects of dihydromyricetin (DHM) on the AGS human gastric cancer cells and their underlying mechanisms. The effects of DHM on AGS cells were evaluated by using 3-(4, 5-di­methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), lactate dehydrogenase, and Annexin V/propidium iodide (PI) double-staining assays. The underlying mechanisms were determined by using quanti­tative real-time polymerase chain reaction.

Effect of human umbilical cord mesenchymal stem cells on endometriotic cell proliferation and apoptosis

L. N. Xu, Lin, N., Xu, B. N., Li, J. B., and Chen, S. Q., Effect of human umbilical cord mesenchymal stem cells on endometriotic cell proliferation and apoptosis, vol. 14, pp. 16553-16561, 2015.

The objective of this study was to observe the effects of human umbilical cord mesenchymal stem cells (UCMSCs) on the proliferation and apoptosis of endometriotic cells. Endometriotic cells and UCMSCs were primarily cultured in vitro. In the experimental group, a UCMSC and endometriotic cell non-contact co-culture system was established. The control group consisted of 1 x 105 endometriotic cells cultured alone. The proliferation and apoptosis of endometriotic cells were respectively detected using the MTT method and flow cytometry.

Study of the radiotherapy sensitization effects and mechanism of capecitabine (Xeloda) against non-small-cell lung cancer cell line A549

J. J. Zhu, Shan, J. J., Sun, L. B., and Qiu, W. S., Study of the radiotherapy sensitization effects and mechanism of capecitabine (Xeloda) against non-small-cell lung cancer cell line A549, vol. 14, pp. 16386-16391, 2015.

The purpose of this study was to explore the radiotherapy sensitization effects and the mechanism of capecitabine (Xeloda) against the non-small-cell lung cancer cell line, A549. γ-[60Co] radiation was used as the intervention method. Proliferative inhibition of capecitabine on A549 cells was determined by the CCK-8 method. The effects of capecitabine on the apoptosis rate and cell cycle distribution of A549 were detected with the flow cytometric method.

Effects of cetuximab combined with afatinib on the expression of KDR and AQP1 in lung cancer

Y. H. Liu and Zhu, W. L., Effects of cetuximab combined with afatinib on the expression of KDR and AQP1 in lung cancer, vol. 14, pp. 16652-16661, 2015.

In this study, we examined the effect of cetuximab (epidermal growth factor receptor monoclonal antibody) combined with afatinib (epidermal growth factor receptorand human epidermal growth factor receptor 2 tyrosine kinase irreversible inhibitor) on the apoptosis of A549 cells and on kinase domain receptor (KDR) and aquaporin 1 (AQP1) expression in A549 cells. A549 cells were cultured in RPMI-1640 and then divided into 4 groups: control group, 1-nM cetuximab group, 25-μM afatinib group, and 1-nM cetuximab + 25-μM afatinib group.

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