Apoptosis

Effects of cetuximab combined with afatinib on the expression of KDR and AQP1 in lung cancer

Y. H. Liu and Zhu, W. L., “Effects of cetuximab combined with afatinib on the expression of KDR and AQP1 in lung cancer”, vol. 14, pp. 16652-16661, 2015.

In this study, we examined the effect of cetuximab (epidermal growth factor receptor monoclonal antibody) combined with afatinib (epidermal growth factor receptorand human epidermal growth factor receptor 2 tyrosine kinase irreversible inhibitor) on the apoptosis of A549 cells and on kinase domain receptor (KDR) and aquaporin 1 (AQP1) expression in A549 cells. A549 cells were cultured in RPMI-1640 and then divided into 4 groups: control group, 1-nM cetuximab group, 25-μM afatinib group, and 1-nM cetuximab + 25-μM afatinib group.

Expression and effect of serum interleukin-24 level on bone marrow mononuclear cells in children with acute leukemia

H. R. Cheng, Wu, B. Q., Chen, L., Zhang, Z. X., and Li, B., “Expression and effect of serum interleukin-24 level on bone marrow mononuclear cells in children with acute leukemia”, vol. 14, pp. 17281-17288, 2015.

To investigate the expression of interleukin-24 (IL-24) in the children with acute leukemia (AL) and its effect on the apoptosis of bone marrow mononuclear cells (BMMNCs) in vitro. Four groups were assessed: acute lymphocytic leukemia (ALL), acute myeloid leukemia (AML), non-leukemia, and healthy groups, 20 children in each group. ELISA was used to measure IL-24 serum level. The bone marrow was taken from patients and controls. BMMNCs were isolated and the DNA was analyzed by glucose electrophoresis. Flow cytometry was used to determine BMMNC apoptosis.