Antifungal activity

Antifungal and antibacterial activities of Petroselinum crispum essential oil

G. A. Linde, Gazim, Z. C., Cardoso, B. K., Jorge, L. F., Tešević, V., Glamoćlija, J., Soković, M., Colauto, N. B., Linde, G. A., Gazim, Z. C., Cardoso, B. K., Jorge, L. F., Tešević, V., Glamoćlija, J., Soković, M., and Colauto, N. B., Antifungal and antibacterial activities of Petroselinum crispum essential oil, vol. 15, p. -, 2016.

Parsley [Petroselinum crispum (Mill.) Fuss] is regarded as an aromatic, culinary, and medicinal plant and is used in the cosmetic, food, and pharmaceutical industries. However, few studies with conflicting results have been conducted on the antimicrobial activity of parsley essential oil. In addition, there have been no reports of essential oil obtained from parsley aerial parts, except seeds, as an alternative natural antimicrobial agent. Also, microorganism resistance is still a challenge for health and food production.

Purification, characterization, and heterologous expression of an antifungal protein from the endophytic Bacillus subtilis strain Em7 and its activity against Sclerotinia sclerotiorum

N. N. Wang, Gao, X. N., Yan, X., Li, Z. P., Kang, Z. S., Huang, L. L., and Han, Q. M., Purification, characterization, and heterologous expression of an antifungal protein from the endophytic Bacillus subtilis strain Em7 and its activity against Sclerotinia sclerotiorum, vol. 14, pp. 15488-15504, 2015.

An antifungal protein exhibiting a high activity against Sclero­tinia sclerotiorum in vivo was purified by ammonium sulfate precipitation, hydrophobic chromatography, and gel filtration chromatography from the culture filtrate of the endophytic Bacillus subtilis strain Em7. The protein was characterized as a β-1,3-1,4-glucanase according to amino acid analysis, and showed excellent properties in thermal stability and acid resistance. At the same time, the antifungal protein was cloned and het­erologously expressed in Escherichia coli BL21.

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