Antifungal activity

Purification, characterization, and heterologous expression of an antifungal protein from the endophytic Bacillus subtilis strain Em7 and its activity against Sclerotinia sclerotiorum

N. N. Wang, Gao, X. N., Yan, X., Li, Z. P., Kang, Z. S., Huang, L. L., and Han, Q. M., “Purification, characterization, and heterologous expression of an antifungal protein from the endophytic Bacillus subtilis strain Em7 and its activity against Sclerotinia sclerotiorum”, vol. 14, pp. 15488-15504, 2015.

An antifungal protein exhibiting a high activity against Sclero­tinia sclerotiorum in vivo was purified by ammonium sulfate precipitation, hydrophobic chromatography, and gel filtration chromatography from the culture filtrate of the endophytic Bacillus subtilis strain Em7. The protein was characterized as a β-1,3-1,4-glucanase according to amino acid analysis, and showed excellent properties in thermal stability and acid resistance. At the same time, the antifungal protein was cloned and het­erologously expressed in Escherichia coli BL21.

Streptomyces ansochromogenes Tur-10 produces a substance with antifungal bioactivity

N. M. Vasconcelos, Fontes, J. M., Lins, M. R. C. R., Bernardo, G. R. B., Araújo, J. M., and Lima, G. M. S., “Streptomyces ansochromogenes Tur-10 produces a substance with antifungal bioactivity”, vol. 14, pp. 5435-5444, 2015.

The increased incidence of fungal infections and the development of drug resistance have led to the search for microorganisms capable of producing bioactive metabolites with antifungal activity. Among these microorganisms, Streptomyces spp are distinguished mainly owing to their potential to secrete bioactive molecules. The aim of this study was to evaluate the production of secondary metabolites by Streptomyces sp TUR-10 against 12 fungal clinical isolates (yeast and filamentous fungi).

A cultured endophyte community is associated with the plant Clerodendrum inerme and antifungal activity

B. Gong, Yao, X. H., Zhang, Y. Q., Fang, H. Y., Pang, T. C., and Dong, Q. L., “A cultured endophyte community is associated with the plant Clerodendrum inerme and antifungal activity”, vol. 14, pp. 6084-6093, 2015.

Fungal endophytes live in the inner tissues of Clerodendrum inerme and may be significant resources for new chemicals in drug discovery. A total of 242 endophytic fungi were recovered from 602 sample segments of C. inerme; 66 were purified.

Diversity and potential application of endophytic bacteria in ginger

T. Chen, Chen, Z., Ma, G. H., Du, B. H., Shen, B., Ding, Y. Q., and Xu, K., “Diversity and potential application of endophytic bacteria in ginger”, vol. 13, pp. 4918-4931, 2014.

Here, 248 endophytic bacterial strains were isolated to assess the distribution and population diversity of endophytic bacteria in ginger plants. A total of 10.4 x 104 to 20.2 x 104 CFU/g fresh weight endophytic bacteria of different growth stages were isolated. Maximum bacterium numbers were obtained at the seedling stage. A total of 107 functional strains were screened, including 17 antibacterial strains and 90 indole acetic acid-producing strains.

Isolation and characterization of genetic variability in bacteria with β-hemolytic and antifungal activity isolated from the rhizosphere of Medicago truncatula plants

J. E. Hernandez-Salmeron, Prieto-Barajas, C. M., Valencia-Cantero, E., Moreno-Hagelsieb, G., and Santoyo, G., “Isolation and characterization of genetic variability in bacteria with β-hemolytic and antifungal activity isolated from the rhizosphere of Medicago truncatula plants”, vol. 13, pp. 4967-4975, 2014.

In the present study, we analyzed the frequency of hemolytic and antifungal activities in bacterial isolates from the rhizosphere of Medicago truncatula plants. Of the 2000 bacterial colonies, 96 showed β-hemolytic activities (frequency, 4.8 x 10-2). Hemolytic isolates were analyzed for their genetic diversity by using random amplification of polymorphic DNA, yielding 88 haplotypes. The similarity coefficient of Nei and Li showed a polymorphic diversity ranging from 0.3 to 1.

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