Annealing control primer system

Identification of differentially expressed genes in a pistillody common wheat mutant using an annealing control primer system

M. L. Liao, Peng, Z. S., Yang, Z. J., Wei, S. H., and Martinek, P., Identification of differentially expressed genes in a pistillody common wheat mutant using an annealing control primer system, vol. 14, pp. 3995-4004, 2015.

HTS-1 is a new kind of pistillody wheat. All or parts of its stamen are transformed into pistils or pistil-like structures, and it has more seed sets per floret than normal wheat under normal cultivation conditions. To investigate the expression divergence in this mutant, an annealing control primer system was used to identify differentially expressed genes (DEGs) in the young spikelets. As a result, three DEGs, including HDB2, HGF2, and HCG4, were detected, with variable expression in HTS-1 and the control.

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