Amylose content

RNA interference-mediated silencing of the starch branching enzyme gene improves amylose content in rice

H. Y. Jiang, Zhang, J., Wang, J. M., Xia, M., Zhu, S. W., and Cheng, B. J., RNA interference-mediated silencing of the starch branching enzyme gene improves amylose content in rice, vol. 12, pp. 2800-2808, 2013.

Amylose and amylopectin are the 2 major components of plant storage starch. The rice starch branching enzyme (RBE) plays an important role in the starch components of rice. In the present study, we selected a specific 195-bp segment from the RBE3 gene to construct hairpin DNA, which was driven by an endosperm-specific high molecular weight glutenin promoter to regulate the biosynthesis of starch. An RNA interference plasmid for the RBE3 gene was constructed to form double-stranded RNA.

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