Alu

Effect of mutations in a simian virus 40 PolyA signal enhancer on green fluorescent protein reporter gene expression

H. G. Wang, Wang, X. F., Jing, X. Y., Li, Z., Zhang, Y., and Lv, Z. J., Effect of mutations in a simian virus 40 PolyA signal enhancer on green fluorescent protein reporter gene expression, vol. 10, pp. 1866-1883, 2011.

Our previous studies have shown that tandem Alu repeats inhibit green fluorescent protein (GFP) gene expression when inserted downstream of the GFP gene in the pEGFP-C1 vector. We found that the 22R sequence (5'-GTGAAAAAAATGCTTTATTTGT-3') from the antisense PolyA (240 bp polyadenylation signal) of simian virus 40, eliminated repression of GFP gene expression when inserted between the GFP gene and the Alu repeats.

A novel polymorphic Alu insertion embedded in a LINE 1 retrotransposon in the human X chromosome (DXS225): identification and worldwide population study

R. Wellerson Pereira, Santos, S. Silva dos, and Pena, S. Danilo Jun, A novel polymorphic Alu insertion embedded in a LINE 1 retrotransposon in the human X chromosome (DXS225): identification and worldwide population study, vol. 5, pp. 63-71, 2006.

We describe a novel polymorphic Alu insertion (DXS225) on the human X chromosome (Xq21.3) embedded into an L1 retrotransposon. The DXS225 polymorphism was genotyped in 684 males from the CEPH Human Genome Diversity Panel. This insertion was found in all regions of the globe, suggesting that it took place before modern humans spread from Africa ca. 100,000 years ago. However, only one Amerindian population (Karitiana) showed this insertion allele, which may have been introduced by European admixture.

Increased gene coverage and Alu frequency in large linkage disequilibrium blocks of the human genome

Y. Wang, Fong, P. Y., Leung, F. C. C., Mak, W., and Sham, P. C., Increased gene coverage and Alu frequency in large linkage disequilibrium blocks of the human genome, vol. 6, pp. 1131-1141, 2007.

The human genome has linkage disequilibrium (LD) blocks, within which single-nucleotide polymorphisms show strong association with each other. We examined data from the International HapMap Project to define LD blocks and to detect DNA sequence features inside of them. We used permutation tests to determine the empirical significance of the association of LD blocks with genes and Alu repeats.

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