Alligator sinensis

Cloning and sequence analysis of the coding sequence of β-actin cDNA from the Chinese alligator and suitable internal reference primers from the β-actin gene

H. N. Zhu, Zhang, S. Z., Zhou, Y. K., Wang, C. L., and Wu, X. B., “Cloning and sequence analysis of the coding sequence of β-actin cDNA from the Chinese alligator and suitable internal reference primers from the β-actin gene”, vol. 14, pp. 12159-12167, 2015.

β-Actin is an essential component of the cytoskeleton and is stably expressed in various tissues of animals, thus, it is commonly used as an internal reference for gene expression studies. In this study, a 1731-bp fragment of β-actin cDNA from Alligator sinensis was obtained using the homology cloning technique. Sequence analysis showed that this fragment contained the complete coding sequence of the β-actin gene (1128 bp), encoding 375 amino acids.

Full-length cDNA cloning and structural characterization of preproinsulin in Alligator sinensis

R. Zhang, Zhang, S. Z., Li, E., Wang, C., Wang, C. L., and Wu, X. B., “Full-length cDNA cloning and structural characterization of preproinsulin in Alligator sinensis”, vol. 13, pp. 8845-8855, 2014.

Insulin is an important endocrine hormone that plays a critical physiological role in regulating metabolism and glucostasis in vertebrates. In this study, the complete cDNA of Alligator sinensis preproinsulin gene was cloned for the first time by reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends methods; the amino acid sequence encoded and protein structure were analyzed.