Aeromonas hydrophila

Hemerythrin is required for Aeromonas hydraphlia to survive in the macrophages of Anguilla japonica

W. B. Zeng, Chen, W. B., Yan, Q. P., Lin, G. F., Qin, Y. X., Zeng, W. B., Chen, W. B., Yan, Q. P., Lin, G. F., and Qin, Y. X., “Hemerythrin is required for Aeromonas hydraphlia to survive in the macrophages of Anguilla japonica”, vol. 15, p. -, 2016.

Survival in host phagocytes is an effective strategy for pathogenic microbes to spread. To understand the mechanisms of Aeromonas hydrophila survival within host macrophages, a library of mini-Tn10 transposon insertion mutants was constructed. The M85 mutant, whose survival in host macrophages was only 23.1% of that of the wild-type (WT) strain, was utilized for further study. Molecular analysis showed that a 756-bp open reading frame (ORF) (GenBank accession No. CP007576) in the M85 mutant was interrupted by mini-Tn10.

A 425 T>C polymorphism in complement C7 association with resistance to Aeromonas hydrophila in grass carp

Y. B. Shen, Zhang, J. B., Fu, J. J., Xu, X. Y., Li, J. L., Wang, R. Q., Xuan, Y. F., Shen, Y. B., Zhang, J. B., Fu, J. J., Xu, X. Y., Li, J. L., Wang, R. Q., Xuan, Y. F., Shen, Y. B., Zhang, J. B., Fu, J. J., Xu, X. Y., Li, J. L., Wang, R. Q., and Xuan, Y. F., “A 425 T>C polymorphism in complement C7 association with resistance to Aeromonas hydrophila in grass carp”, vol. 15, p. -, 2016.

Aeromonas hydrophila, a widespread bacterium in the aquatic environment, causes hemorrhagic septicemia in fish. In the last decade, the disease has caused mass mortalities and tremendous economic loss in cultured fish. The complement component C7 is a terminal component of complement that interacts in a sequence of polymerization reactions with other terminal complement components to form a membrane attack complex. The formation of the membrane attack complex creates a pore in the membranes of certain pathogen that can lead to their death.

Differential expression of interleukin-12 p35 and p40 subunits in response to Aeromonas hydrophila and Aquareovirus infection in grass carp, Ctenopharyngodon idella

N. P. Pandit, Shen, Y. B., Xu, X. Y., Yu, H. Y., Wang, W. J., Wang, R. Q., Xuan, Y. F., and Li, J. L., “Differential expression of interleukin-12 p35 and p40 subunits in response to Aeromonas hydrophila and Aquareovirus infection in grass carp, Ctenopharyngodon idella”, vol. 14, pp. 1169-1183, 2015.

The grass carp (Ctenopharyngodon idella) aquaculture industry in Asia is prone to bacterial and viral hemorrhagic diseases. Effective adjuvants for vaccine formulation are the need of the hour for control of these diseases and long-term sustainability of grass carp farming. In this study, the involvement of interleukin-12 (IL-12) from grass carp (gcIL‑12) in anti-bacterial and anti-viral immune responses was demonstrated via expression profiles of gcIL-12 subunits in immune tissues of the fish, following infection by Aeromonas hydrophila and Aquareovirus.

FlgN plays important roles in the adhesion of Aeromonas hydrophila to host mucus

X. Jiang, Qin, Y. X., Lin, G. F., Huang, L., Huang, B., Huang, W. S., and Yan, Q. P., “FlgN plays important roles in the adhesion of Aeromonas hydrophila to host mucus”, vol. 14, pp. 6376-6386, 2015.

Adhesion to the host mucus is a crucial step in the early infection stage of pathogenic bacteria. To investigate the mechanisms of the adhesion of Aeromonas hydrophila to its host mucus, a mutant library was constructed using the mini-Tn10 transposon mutagenesis system. Of 276 individual colonies, the mutant strain with the most attenuated adhesion ability in this study was screened out and designated A77.

Role of MshQ in MSHA pili biosynthesis and biofilm formation of Aeromonas hydrophila

Y. X. Qin, Yan, Q. P., Mao, X. X., Chen, Z., and Su, Y. Q., “Role of MshQ in MSHA pili biosynthesis and biofilm formation of Aeromonas hydrophila”, vol. 13, pp. 8982-8996, 2014.

Biofilm formation of pathogen bacterium is currently one of the most widely studied topics; however, little is known regarding pathogen bacteria biofilms in aquaculture. Aeromonas hydrophila is a representative species of the genus Aeromonas, which has been recognized as a common pathogen, is associated with many diseases in aquatic animals, and causes significant mortality. The objectives of this study are i) to confirm that A.

Molecular characterization of a cytosolic manganese superoxide dismutase from the Chinese mitten crab, Eriocheir sinensis

D. X. Zhao, Chen, L. Q., Qin, J. G., Qin, C. J., Zhang, H., Wu, P., and Li, E. C., “Molecular characterization of a cytosolic manganese superoxide dismutase from the Chinese mitten crab, Eriocheir sinensis”, vol. 13, pp. 9429-9442, 2014.

A cytosolic manganese superoxide dismutase gene (Es-cMnSOD) was cloned from the Chinese mitten crab Eriocheir sinensis, using reverse transcription-polymerase chain reaction and the rapid amplification of cDNA ends. The open reading frame of Es-cMnSOD is 867 bp in length and encodes a 288-amino acid protein without a signal peptide. The calculated molecular mass of the translated protein of Es-cMnSOD is 31.43 kDa, with an estimated isoelectric point of 6.30.

Molecular characterization, expression, and immunological response analysis of the TWEAK and APRIL genes in grass carp, Ctenopharyngodon idella

N. P. Pandit, Shen, Y. B., Chen, Y., Wang, W. J., and Li, J. L., “Molecular characterization, expression, and immunological response analysis of the TWEAK and APRIL genes in grass carp, Ctenopharyngodon idella”, vol. 13, pp. 10105-10120, 2014.

TWEAK and APRIL are important members of the TNF superfamily, which play a crucial role in several diseases. Here, we describe the identification of grass carp (Ctenopharyngodon idella) homologs of TWEAK and APRIL (designated gcTWEAK and gcAPRIL, respectively) and their response to Aeromonas hydrophila and Aquareovirus infection. The gcTWEAK cDNA sequence contains 2273 bases with an open reading frame of 753 bases encoding 250-amino acid residues.

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