Adventitious shoots induction

Overexpression of the CmACS-3 gene in melon causes abnormal pollen development

H. Zhang and Luan, F., Overexpression of the CmACS-3 gene in melon causes abnormal pollen development, vol. 14, pp. 10433-10443, 2015.

Sexual diversity expressed by the Curcurbitaceae family is a primary example of developmental plasticity in plants. Most melon genotypes are andromonoecious, where an initial phase of male flowers is followed by a mixture of bisexual and male flowers. Over-expression of the CmACS-3 gene in melon plants showed an increased number of flower buds, and increased femaleness as demonstrated by a larger number bisexual buds.

An efficient regeneration protocol for Agrobacterium-mediated transformation of melon (Cucumis melo L.)

H. J. Zhang, Gao, P., Wang, X. Z., and Luan, F. S., An efficient regeneration protocol for Agrobacterium-mediated transformation of melon (Cucumis melo L.), vol. 13, pp. 54-63, 2014.

An efficient selection and plant regeneration protocol for Agrobacterium-mediated transformation, using cotyledon node zone-stem connection region of melon, has been developed. The new Agrobacterium-mediated transformation methodology, independent of organ culture, used the entire germinated seed as explants. The transformation system was maximized to maintain the integrity of melon itself, thus avoiding the limitations of traditional tissue culture methods. The transformation was carried out under a non-sterile environment.

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