β-actin

Molecular characterization and expression analysis of the β-actin gene from the ridgetail white prawn Exopalaemon carinicauda

J. P. Liang, Wang, Y., Ge, Q. Q., Li, J. T., Liu, P., Li, J., Nie, G. X., Liang, J. P., Wang, Y., Ge, Q. Q., Li, J. T., Liu, P., Li, J., Nie, G. X., Liang, J. P., Wang, Y., Ge, Q. Q., Li, J. T., Liu, P., Li, J., and Nie, G. X., Molecular characterization and expression analysis of the β-actin gene from the ridgetail white prawn Exopalaemon carinicauda, vol. 15, p. -, 2016.

Actin is a highly conserved protein that is found in all eukaryotic cells, and has been widely used as an internal control gene in gene expression studies. In this study, we cloned an actin gene (named Ecβ-actin) from Exopalaemon carinicauda and determined its expression levels. The full-length cDNA of Ecβ-actin was 1335 bp long, comprising a 1131-bp ORF encoding 376 amino acids, a 65-bp 5'-UTR, and a 139-bp 3'-UTR with a poly(A) tail.

Cloning and sequence analysis of the coding sequence of β-actin cDNA from the Chinese alligator and suitable internal reference primers from the β-actin gene

H. N. Zhu, Zhang, S. Z., Zhou, Y. K., Wang, C. L., and Wu, X. B., Cloning and sequence analysis of the coding sequence of β-actin cDNA from the Chinese alligator and suitable internal reference primers from the β-actin gene, vol. 14, pp. 12159-12167, 2015.

β-Actin is an essential component of the cytoskeleton and is stably expressed in various tissues of animals, thus, it is commonly used as an internal reference for gene expression studies. In this study, a 1731-bp fragment of β-actin cDNA from Alligator sinensis was obtained using the homology cloning technique. Sequence analysis showed that this fragment contained the complete coding sequence of the β-actin gene (1128 bp), encoding 375 amino acids.

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