Acetic acid

An alternative cetyltrimethylammonium bromide-based protocol for RNA isolation from blackberry (Rubus L.)

Q. Chen, Yu, H. W., Wang, X. R., Xie, X. L., Yue, X. Y., and Tang, H. R., An alternative cetyltrimethylammonium bromide-based protocol for RNA isolation from blackberry (Rubus L.), vol. 11. pp. 1773-1782, 2012.

Isolation of high-quality RNA free of contaminants, such as polyphenols, proteins, plant secondary metabolites, and genomic DNA from plant tissues, is usually a challenging but crucial step for molecular analysis. We developed a novel protocol based on the cetyltrimethylammonium bromide method to isolate high-quality RNA from blackberry plant tissues, especially fruits. Most DNA was removed when acetic acid was utilized, before RNA precipitation. Thus, lithium chloride, a reagent widely used for RNA purification, was not needed. The isolation time was shortened to less than 3 h.

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