454 Pyrosequencing

Development and characterization of novel microsatellite markers for Ginkgo biloba using 454 pyrosequencing

Q. Zhou, Wang, X. X., Xu, M., Cao, F. L., Yu, F. X., Xu, L. A., Zhou, Q., Wang, X. X., Xu, M., Cao, F. L., Yu, F. X., Xu, L. A., Zhou, Q., Wang, X. X., Xu, M., Cao, F. L., Yu, F. X., and Xu, L. A., Development and characterization of novel microsatellite markers for Ginkgo biloba using 454 pyrosequencing, vol. 15, p. -, 2016.

As a “living fossil” that is used to understand the evolutionary history of seed plants, Ginkgo biloba is a well-known multipurpose tree with edible seeds, medicinal properties, and ornamental value, but little is known about its genetic diversity. Microsatellite, or simple sequence repeat (SSR), markers have proven to be powerful tools for genetic studies of plants. In this study, we isolated 30 novel polymorphic microsatellite loci in G. biloba using 454 pyrosequencing. The characteristics of these loci were tested with 48 cultivars.

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