16S rRNA

Phylogenetic analysis of endophytic bacterial isolates from leaves of the medicinal plant Trichilia elegans A. Juss. (Meliaceae)

S. A. Rhoden, Garcia, A., Silva, M. C. Santos e, Azevedo, J. L., and Pamphile, J. A., Phylogenetic analysis of endophytic bacterial isolates from leaves of the medicinal plant Trichilia elegans A. Juss. (Meliaceae), vol. 14, pp. 1515-1525, 2015.

Various organisms such as fungi and bacteria can live inside plants, inhabiting the aerial parts (primarily the leaves) without causing damage. These microorganisms, called endophytes, produce an extensive variety of compounds that can be useful for medical and agronomic purposes. Trichilia elegans A. Juss., belonging to the Meliaceae family, shows wide dispersion in South America, and phytochemical analyses from these plants and endophyte isolates have shown biological activity.

Qualitative analysis of the vaginal microbiota of healthy cattle and cattle with genital-tract disease

N. F. Rodrigues, Kästle, J., Coutinho, T. J. D., Amorim, A. T., Campos, G. B., Santos, V. M., Marques, L. M., Timenetsky, J., and de Farias, S. T., Qualitative analysis of the vaginal microbiota of healthy cattle and cattle with genital-tract disease, vol. 14, pp. 6518-6528, 2015.

The microbial community of the reproductive appara­tus, when known, can provide information about the health of the host. Metagenomics has been used to characterize and obtain genetic infor­mation about microbial communities in various environments and can relate certain diseases with changes in this community composition. In this study, samples of vaginal surface mucosal secretions were col­lected from five healthy cows and five cows that showed symptoms of reproductive disorders.

Genetic variability and phylogenetic aspects in species of the genus Macrobrachium

A. L. Guerra, Lima, A. V. B., Júnior, R. V. Lucato, Chiachio, M. C., Taddei, F. G., and Castiglioni, L., Genetic variability and phylogenetic aspects in species of the genus Macrobrachium, vol. 13, pp. 3646-3655, 2014.

The genus Macrobrachium includes prawns, which are widely distributed in lakes, floodplains, and rivers in tropical and subtropical regions of South America. This genus presents nearly 210 known species with great ecological and economic importance. However, few studies are related to the biology of these crustaceans. In this study, we analyzed the genetic variability and phylogenetic relationship between Macrobrachium amazonicum and Macrobrachium jelskii, which are closely related species.

Microbial community and performance of slaughterhouse wastewater treatment filters

M. I. Stets, Etto, R. M., Galvão, C. W., Ayub, R. A., Cruz, L. M., Steffens, M. B. R., and Barana, A. C., Microbial community and performance of slaughterhouse wastewater treatment filters, vol. 13, pp. 4444-4455, 2014.

The performance of anaerobic filter bioreactors (AFs) is influenced by the composition of the substrate, support medium, and the microbial species present in the sludge. In this study, the efficiency of a slaughterhouse effluent treatment using three AFs containing different support media was tested, and the microbial diversity was investigated by amplified ribosomal DNA restriction analysis and 16S rRNA gene sequencing.

Analysis of Antarctic proteobacteria by PCR fingerprinting and screening for antimicrobial secondary metabolites

L. - H. Lee, Cheah, Y. - K., Syakima, A. M. Nurul, Shiran, M. S., Tang, Y. - L., Lin, H. - P., and Hong, K., Analysis of Antarctic proteobacteria by PCR fingerprinting and screening for antimicrobial secondary metabolites, vol. 11, pp. 1627-1641, 2012.

Fifty-seven proteobacterium species were successfully isolated from soils of Barrientos Island of the Antarctic using 11 different isolation media. Analysis of 16S rDNA sequencing of these isolates showed that they belonged to eight different genera, namely Bradyrhizobium, Sphingomonas, Methylobacterium, Caulobacter, Paracoccus, Ralstonia, Rhizobium, and Staphylococcus. All isolates were studied for capability of producing antimicrobial and antifungal secondary metabolites using high-throughput screening models.

Simple and inexpensive DNA extraction protocol for studying the bacterial composition of sludges used in microbial fuel cells

B. Canto-Canché, Tzec-Simá, M., Vázquez-Loría, J. I., Espadas-Álvarez, H., Chí-Manzanero, B. H., Rojas-Herrera, R., Valdez-Ojeda, R., and Alzate-Gaviria, L., Simple and inexpensive DNA extraction protocol for studying the bacterial composition of sludges used in microbial fuel cells, vol. 12. pp. 282-292, 2013.

Bacteria oxidize organic matter and nutrients to produce electric energy in microbial fuel cells (MFC) - a technology of increasing importance because of its sustainability. To improve the performance of MFCs, it is necessary not only to gain a better understanding of MFC engineering designs, but also to improve the understanding of the composition of the microbial communities in MFCs.

Phylogenetic relationships among Octopodidae species in coastal waters of China inferred from two mitochondrial DNA gene sequences

Z. M. Lü, Cui, W. T., Liu, L. Q., Li, H. M., and Wu, C. W., Phylogenetic relationships among Octopodidae species in coastal waters of China inferred from two mitochondrial DNA gene sequences, vol. 12, pp. 3755-3765, 2013.

Octopus in the family Octopodidae (Mollusca: Cephalopoda) has been generally recognized as a "catch-all" genus. The monophyly of octopus species in China's coastal waters has not yet been studied. In this paper, we inferred the phylogeny of 11 octopus species (family Octopodidae) in China's coastal waters using nucleotide sequences of two mitochondrial DNA genes: cytochrome c oxidase subunit I (COI) and 16S rRNA.

Culture-independent analysis of endophytic bacterial communities associated with Brazilian sugarcane

G. S. Magnani, Cruz, L. M., Weber, H., Bespalhok, J. C., Daros, E., Baura, V., Yates, M. G., Monteiro, R. A., Faoro, H., Pedrosa, F. O., and Souza, E. M., Culture-independent analysis of endophytic bacterial communities associated with Brazilian sugarcane, vol. 12, pp. 4549-4558, 2013.

Sugarcane is an economically important culture in Brazil. Endophytic bacteria live inside plants, and can provide many benefits to the plant host. We analyzed the bacterial diversity of sugarcane cultivar RB-72454 by cultivation-independent techniques. Total DNA from sugarcane stems from a commercial plantation located in Paraná State was extracted. Partial 16S rRNA genes were amplified and sequenced for library construction. Of 152 sequences obtained, 52% were similar to 16S rRNA from Pseudomonas sp, and 35.5% to Enterobacter sp.

Diversity of endophytic bacteria in Brazilian sugarcane

G. S. Magnani, Didonet, C. M., Cruz, L. M., Picheth, C. F., Pedrosa, F. O., and Souza, E. M., Diversity of endophytic bacteria in Brazilian sugarcane, vol. 9, pp. 250-258, 2010.

Endophytic bacteria live inside plant tissues without causing disease. Studies of endophytes in sugarcane have focused on the isolation of diazotrophic bacteria. We examined the diversity of endophytic bacteria in the internal tissues of sugarcane stems and leaves, using molecular and biochemical methods. Potato-agar medium was used to cultivate the endophytes; 32 isolates were selected for analysis. DNA was extracted and the 16S rRNA gene was partially sequenced and used for molecular identification.

Subscribe to 16S rRNA