16S rDNA

Genetic variability in isolates of Chromobacterium violaceum from pulmonary secretion, water, and soil

A. C. Santini, Magalhães, J. T., Cascardo, J. C. M., Corrêa, R. X., Santini, A. C., Magalhães, J. T., Cascardo, J. C. M., Corrêa, R. X., Santini, A. C., Magalhães, J. T., Cascardo, J. C. M., and Corrêa, R. X., Genetic variability in isolates of Chromobacterium violaceum from pulmonary secretion, water, and soil, vol. 15, p. -, 2016.

Chromobacterium violaceum is a free-living Gram-negative bacillus usually found in the water and soil in tropical regions, which causes infections in humans. Chromobacteriosis is characterized by rapid dissemination and high mortality. The aim of this study was to detect the genetic variability among C. violaceum type strain ATCC 12472, and seven isolates from the environment and one from a pulmonary secretion from a chromobacteriosis patient from Ilhéus, Bahia.

Genetic diversity of Angiopolybia pallens (Lepeletier) (Hymenoptera, Vespidae, Polistinae) explained by the disjunction of South American rainforests

A. F. Carvalho, Santos, G. M. M., Menezes, R. S. T., and Costa, M. A., Genetic diversity of Angiopolybia pallens (Lepeletier) (Hymenoptera, Vespidae, Polistinae) explained by the disjunction of South American rainforests, vol. 13, pp. 89-94, 2014.

Angiopolybia pallens is a swarm-founding wasp that occurs in Atlantic and Amazonian rainforests, which have been isolated from each other by a large arid corridor in central Brazil since the Tertiary. In this study, we used data from polymerase chain reaction-restriction fragment length polymorphism of 16S rDNA to infer some aspects of the genetic diversity and evolutionary history of this social wasp population. Samples collected across the species range in both rainforests showed remarkable differences that separated them into two distinct haplogroups.

Evaluation of the biodegradability of petroleum in microcosm systems by using mangrove sediments from Camamu Bay, Bahia, Brazil

A. C. F. Santos, Rezende, R. P., Brendel, M., Souza, S. S., Gonçalves, A. C. S., and Dias, J. C. T., Evaluation of the biodegradability of petroleum in microcosm systems by using mangrove sediments from Camamu Bay, Bahia, Brazil, vol. 13, pp. 2048-2059, 2014.

We investigated the biodegradability of oil in mangrove sediment from Camamu Bay and measured its effect on the bacterial community. Microcosms of mangrove sediment were contaminated with 0.1, 0.5, 1, 2, and 5% (w/v) oil, and the microbial activity was compared to that in uncontaminated sediment. The evolution of CO2 and gas chromatography showed the mineralization of oil compounds, which could reach 100%. Bacterial diversity was determined by polymerase chain reaction using a set of primers for the V3 and V6-V8 regions of 16S rDNA.

Diversity of endophytic bacteria in Caragana microphylla grown in the desert grassland of the Ningxia Hui Autonomous Region of China

J. X. Dai, Liu, X. M., and Wang, Y. J., Diversity of endophytic bacteria in Caragana microphylla grown in the desert grassland of the Ningxia Hui Autonomous Region of China, vol. 13, pp. 2349-2358, 2014.

The diversity of endophytic bacteria in the sand-fixation plant Caragana microphylla was investigated by amplified rDNA restriction analysis and by sequence and phylogenetic comparisons of the 16S rRNA genes. A total of 24, 19, and 17 operational taxonomic units were identified from 16S rDNA libraries of the plant roots, stems and leaves, respectively. Homology analysis revealed a 92-100% identity of bacterial 16S rDNA sequences compared with those in the GenBank database.

Identification of actinomycete communities in Antarctic soil from Barrientos Island using PCR-denaturing gradient gel electrophoresis

L. Learn-Han, Yoke-Kqueen, C., Shiran, M. S., Vui-Ling, C. M. W., Nurul-Syakima, A. M., Son, R., and Andrade, H. M., Identification of actinomycete communities in Antarctic soil from Barrientos Island using PCR-denaturing gradient gel electrophoresis, vol. 11, pp. 277-291, 2012.

The diversity of specific bacteria taxa, such as the actinomycetes, has not been reported from the Antarctic island of Barrientos. The diversity of actinomycetes was estimated with two different strategies that use PCR-denaturing gradient gel electrophoresis. First, a PCR was applied, using a group-specific primer that allows selective amplification of actinomycete sequences. Second, a nested-PCR approach was used that allows the estimation of the relative abundance of actinomycetes within the bacterial community.

Genetic diversity of Burkholderia (Proteobacteria) species from the Caatinga and Atlantic rainforest biomes in Bahia, Brazil

A. C. Santini, Santos, H. R. M., Gross, E., and Corrêa, R. X., Genetic diversity of Burkholderia (Proteobacteria) species from the Caatinga and Atlantic rainforest biomes in Bahia, Brazil, vol. 12, pp. 655-664, 2013.

The genus Burkholderia (β-Proteobacteria) currently comprises more than 60 species, including parasites, symbionts and free-living organisms. Several new species of Burkholderia have recently been described showing a great diversity of phenotypes. We examined the diversity of Burkholderia spp in environmental samples collected from Caatinga and Atlantic rainforest biomes of Bahia, Brazil. Legume nodules were collected from five locations, and 16S rDNA and recA genes of the isolated microorganisms were analyzed.

Subscribe to 16S rDNA