PHYLOGENETIC RELATIONSHIPS OF TRICHODERMA HARZIANUM ISOLATED FROM PADDY FIELD BASED ON SEQUENCE ANALYSIS

Abstract

The aim of this study was to determine whether sequence analysis of internal transcribed spacer-1 (ITS1) region of the ribosomal DNA can be used to detect phylogenetic relationships of Trichoderma isolates obtained from paddy field. ITS 1 of the rDNA of Trichoderma isolates was amplified by polymerase chain reaction (PCR) using universal primers (ITS1). PCR products were purified and these purified products were used to amplify the ITS region of Trichoderma isolates. The amplified DNA was sequenced and aligned against ex-type strain sequences from Tricho BLAST and established Trichoderma taxonomy. The ITS-1 region sequences have been used as the reference’s sequence and may be used for future study involving the identification and taxonomy of Trichoderma harzianum. Amplification of ITS1 region of the rDNA has showed potential as a rapid technique for identifying Trichoderma harzianum successfully.