URIC ACID DETERMINATION USING NANOASSAYS AND COMPARISON WITH CONVENTIONAL METHODS

Abstract

The urinary uric acid concentration (mg/24h) was indirectly determined using a sophisticated, chemically based nano-reagent method relying on differential pulsed polarography (DPP) measurement on a Dropping mercury electrode (DME), and the results were compared with spectroscopic methods. A comprehensive study of urinary uric acid concentration (mg/24h) was conducted using this nano-prepared Folin-Denis reagent, which relies on the reduction wavelength of a Folin-Denis nano-reagent prepared in the presence of uric acid. This reduction reaction with uric acid in the presence of a base resulted in two distinct reduction waves: one with a reduction potential of -0.507 V and the other with a reduction potential of -0.64 V, on a calomel electrode after applying a potential between -0.2 and -0.8 V. The integration of carbon nanotubes and nano-reagents has shown improved sensitivity, providing a robust electrochemical platform for monitoring uric acid levels associated with kidney function.