Research Article

Intestinal Dysbiosis Increases the Incidence of Malignant Melanoma in Mice Model

Published: November 29, 2017
Genet. Mol. Res. 16(4): gmr16039840 DOI: 10.4238/gmr16039840

Abstract

 Antibiotic-induced disruption of the intestinal microbiota
has serious consequences for human physiology. We conducted a
microbial dysbiosis animal model to study and directly provide evidence
for microbial dysbiosis contribution to malignant melanoma animal
model. Females C57BL/6 wild-type (WT) mice injected with a limiting
threshold number of B16/F1 melanoma cells and the tumor development
was observed with and without probiotics treatment. Treatment with
probiotic could restore microbiota diversity and indirectly effect the
tumor incidence. In microbial dysbiosis group tumor incidence was
83.33%. Also, the expression level of inflammatory proteins NFkB-p65;
IL-6; and STAT-3 were remarkably enhanced in microbial dysbiosis
group compared to probiotic treated group. Altogether, our findings
demonstrated that microbial dysbiosis can strike the balance between
immunity and tumorigenesis, and increase the incidence of malignant
melanoma in mice. Probiotic treatment significantly reduced tumor
incidence.
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